Glutathione Peroxidase (GSH-Px) Activity Assay Kit

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中文名称：谷胱甘肽过氧化物酶（GSH-Px）比色法测试盒

货号：E-BC-K096-S
文献（163）
说明书

说明书

Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Price: ¥680.00 ¥380.00

Size:

100 Assays (48 samples) 50 Assays (24 samples)

样本类型： 血清（浆）;动植物组织;细胞;培养液
检测仪器及波长： 紫外-可见光分光光度计（412 nm）
研究领域： 氧化应激;植物生理系列;铁死亡

该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M

说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证

根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮计算

检测原理	GSH-Px可以促进过氧化氢（H₂O₂）与还原型谷胱甘肽（GSH）反应生成H₂O及氧化型谷胱甘肽（GSSG），反应式如下图。通过测定底物GSH在单位时间内被氧化的速度来表示酶的活力，反应剩余的GSH与二硫代双二硝基苯甲酸（DTNB）作用生成的黄色硫代硝基苯甲酸阴离子（TNB），测定该阴离子的浓度来计算出GSH的减少量。由于GSH在没有酶的条件下也能进行氧化（非酶反应），所以最后计算此酶活力时，必须扣除非酶反应引起的GSH减少的那部分。
指标简称	GSH-Px
灵敏度	12.65 U
检测范围	12.65-387 U
精密度	批间差：9.3% \| 批内差：4.9%
样本量	0.1-0.2 mL
检测时间	1 h 10 min
检测仪器及波长	紫外-可见光分光光度计（412 nm）
保存条件	该产品可在 2-8°C避光条件下保存6个月。
有效期	6个月

Klotho ameliorates the onset and progression of cataract via suppressing oxidative stress and inflammation in the lens in streptozotocin-induced diabetic rats

IF: 3.943

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2020)

DOI：10.1016/j.intimp.2020.106582

PMID：32438076

Reactivity：Rat

Sample Type：Tissue homogenate
Overexpression of ethylene response factor ERF96 gene enhances selenium tolerance in Arabidopsis

IF: 3.720

Journal： PLANT PHYSIOLOGY AND BIOCHEMISTRY (2020)

DOI：10.1016/j.plaphy.2020.02.024

PMID：32097848

Reactivity：Arabidopsis thaliana

Sample Type：Plant?Tissue
Quercetin attenuated oxidative DNA damage through NRF2 signaling pathway in rats with DMH induced colon carcinogenesis

IF: 3.647

Journal： LIFE SCIENCES (2020)

DOI：10.1016/j.lfs.2020.117584

PMID：32220623

Reactivity：Rat

Sample Type：Tissue homogenate
By reducing oxidative stress, naringenin mitigates hyperglycaemia-induced upregulation of hepatic nuclear factor erythroid 2-related factor 2 protein

IF: 2.571

Journal： JOURNAL OF PHARMACY AND PHARMACOLOGY (2020)

DOI：10.1111/jphp.13319

PMID：32628779

Reactivity：Human

Sample Type：Cell lysate
Bredemolic Acid Improves Cardiovascular Function and Attenuates Endothelial Dysfunction in Diet-Induced Prediabetes: Effects on Selected Markers

IF: 2.538

Journal： Cardiovascular Therapeutics (2020)

DOI：10.1155/2020/1936406

PMID：32117470

Reactivity：Rat

Sample Type：Plasma,Tissue homogenate
Cardio-protective effects of a dioxidovanadium(V) complex in male sprague–dawley rats with streptozotocin-induced diabetes

IF: 2.479

Journal： BIOMETALS (2020)

DOI：10.1007/s10534-020-00270-0

PMID：33206308

Reactivity：Rat

Sample Type：Tissue homogenate(Heart)
Quercetin supplemented casein-based extender improves the post-thaw quality of rooster semen

IF: 2.287

Journal： CRYOBIOLOGY (2020)

DOI：10.1016/j.cryobiol.2020.04.010

PMID：32437678

Reactivity：Chicken

Sample Type：Sperm
Expression of α-Klotho Is Downregulated and Associated with Oxidative Stress in the Lens in Streptozotocin-induced Diabetic Rats

IF: 1.754

Journal： CURRENT EYE RESEARCH (2020)

DOI：10.1080/02713683.2020.1805768

PMID：32744464

Reactivity：Rat

Sample Type：Tissue homogenate
The effects of chrysin on lipopolysaccharide-induced sepsis in rats

IF: 1.662

Journal： JOURNAL OF FOOD BIOCHEMISTRY (2020)

DOI：10.1111/jfbc.13359

PMID：32614079

Reactivity：Rat

Sample Type：lung, liver, kidney
Which Distant Organ is Most Affected by Lower Extremity Ischemia-Reperfusion?

IF: 1.125

Journal： ANNALS OF VASCULAR SURGERY (2020)

DOI：10.1016/j.avsg.2020.01.008

PMID：31927058

Reactivity：Rat

Sample Type：Tissue homogenate

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