Goat Anti-Human IgM(peroxidase/HRP conjugated)-Elabscience伊莱瑞特【官网】

Goat Anti-Human IgM(peroxidase/HRP conjugated)

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货号：E-AB-1009
文献（18）
说明书

说明书

Goat Anti-Human IgM(peroxidase/HRP conjugated)

Price: ¥945.00 ¥240.00 ¥145.00 ¥85.00 ¥1200.00

Size:

500 μL 120 μL 60 μL 30 μL 1 mL

宿主： Goat
应用性： WB;ELISA

Source	Goat
Immunogen	Human IgM
Application	WB, ELISA
Recommended Dilution	WB 1:2000-5000(Enhanced chemiluminescent detection) WB 1:300-1500(DAB detection) Direct ELISA 1:3000-5000(TMB detection)
Concentration	0.5 mg/mL
Clonality	Polyclonal
Purification	Affinity purification
Conjugation	HRP
Storage Instructions	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Storage Buffer	PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4

Defective kinase activity of IKKα leads to combined immunodeficiency and disruption of immune tolerance in humans

IF: 14.700

Journal： Nature Communications (2024)

DOI：10.1038/s41467-024-54345-4

Reactivity：Human

Sample Type：serum
Natural antibodies to polysaccharide capsules enable Kupffer cells to capture invading bacteria in the liver sinusoids

IF: 12.600

Journal： JOURNAL OF EXPERIMENTAL MEDICINE (2024)

DOI：10.1084/jem.20240735

Reactivity：Human

Sample Type：serum
IGFBP7 is a key component of the senescence-associated secretory phenotype (SASP) that induces senescence in healthy cells by modulating the insulin, IGF, and activin A pathways

IF: 8.200

Journal： Cell Communication and Signaling (2024)

DOI：10.1186/s12964-024-01921-2

Reactivity：Human
Potential anti-liver cancer targets and mechanisms of kaempferitrin based on network pharmacology, molecular docking and experimental verification

IF: 7.000

Journal： COMPUTERS IN BIOLOGY AND MEDICINE (2024)

DOI：10.1016/j.compbiomed.2024.108693

Reactivity：Mouse,Human

Sample Type：SMMC-7721 cell-Xenograft
Hydroxyurea inhibits proliferation and stimulates apoptosis through inducible nitric oxide synthase in erythroid cells

IF: 6.900

Journal： BIOMEDICINE & PHARMACOTHERAPY (2024)

DOI：10.1016/j.biopha.2024.117723

Reactivity：Human
Bisphenol S exposure interrupted human embryonic stem cell derived cardiomyocytes differentiation through ER-NF-κB/ERK signaling pathway

IF: 6.200

Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024)

DOI：10.1016/j.ecoenv.2024.117576

Reactivity：Human
Meroterpenoids from Marine Sponge Hyrtios sp. and Their Anticancer Activity against Human Colorectal Cancer Cells

IF: 4.900

Journal： Marine Drugs (2024)

DOI：10.3390/md22040183

Reactivity：Human
Formononetin protects against Aspergillus fumigatus Keratitis: Targeting inflammation and fungal load

IF: 4.800

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)

DOI：10.1016/j.intimp.2024.112046

Reactivity：Mouse,Human
Transdermal Delivery of Recombinant Human Growth Hormone by Liposomal Gel for Skin Photoaging Therapy

IF: 4.600

Journal： ACS Applied Bio Materials (2024)

DOI：10.1021/acsabm.4c00391

Reactivity：Human
SLC45A4 is involved in malignant progression of ovarian cancer through glycolytic metabolic reprogramming

IF: 3.800

Journal： Scientific Reports (2024)

DOI：10.1038/s41598-024-74249-z

Reactivity：Human

Q1:该如何选择二抗？

二抗根据一抗的来源和客户的实验需求来选择。
先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。
标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。
ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色；
WB主要选择HRP标记的二抗，用ECL底物发光液显色；
IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色；
IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。