Goat Anti-Mouse IgG
Price:
- 宿主： Goat
- 应用性： In WB, IF,IP,FCM, etc., can be used as IgG controls
| Source | Goat |
| Immunogen | Mouse IgG |
| Application | In WB, IF,IP,FCM, etc., can be used as IgG controls |
| Recommended Dilution | Other When the antibody is labeled, for example, with HRP, FITC, BIOTIN, etc, BSA and NaN3 cannot be contained in reagents. |
| Concentration | 1 mg/mL |
| Clonality | Polyclonal |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage Instructions | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4 |
-
A Novel Drug Candidate for Sepsis Targeting Heparanase by Inhibiting Cytokine Storm
IF: 14.300Journal： Advanced Science (2024)Reactivity：MouseSample Type：Lung,kidney,spleens tissue -
ZO-1 boosts the in vitro self-renewal of pre-haematopoietic stem cells from OCT4-reprogrammed human hair follicle mesenchymal stem cells through cytoskeleton remodeling
IF: 7.100 -
NLRP3-mediated IL-1β in regulating the imbalance between Th17 and Treg in experimental autoimmune prostatitis
IF: 3.800Journal： Scientific Reports (2024)Reactivity：MouseSample Type：prostate tissue -
Phosphoethanolamine cytidylyltransferase ameliorates mitochondrial function and apoptosis in hepatocytes in T2DM in?vitro
IF: 6.500Journal： JOURNAL OF LIPID RESEARCH (2023)PMID：36716821Reactivity：Human,MouseSample Type：liver tissue -
Low-dose spironolactone combats dyslipidemia and hepatic inflammation by modulating PCSK9 in rat model of polycystic ovarian syndrome
IF: 3.800Journal： TOXICOLOGY AND APPLIED PHARMACOLOGY (2023)PMID：37328115Reactivity：RatSample Type：hepatic -
Glabridin Inhibits Aspergillus fumigatus Growth and Alleviate Inflammation Mediated by Dectin-2 and NLRP3 Inflammasome
IF: 2.000 -
Gut Microflora Modulates Th17/Treg Cell Differentiation in Experimental Autoimmune Prostatitis via the Short-Chain Fatty Acid Propionate
IF: 8.786 -
Membrane Attack Complex C5b-9 Promotes Renal Tubular Epithelial Cell Pyroptosis in Trichloroethylene-Sensitized Mice
IF: 5.988 -
Tilianin Reduces Apoptosis via the ERK/EGR1/BCL2L1 Pathway in Ischemia/Reperfusion-Induced Acute Kidney Injury Mice
IF: 5.988 -
The roles of metastasis-related proteins in the development of giant cell tumor of bone, osteosarcoma and Ewing’s sarcoma
IF: 1.285
Q1:该如何选择二抗？
二抗根据一抗的来源和客户的实验需求来选择。
先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。
标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。
ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色；
WB主要选择HRP标记的二抗，用ECL底物发光液显色；
IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色；
IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。
