Goat Anti-Mouse IgG(H+L)(Elab Fluor® 488 conjugated)（E-AB-1056）|-Elabscience伊莱瑞特【官网】

Goat Anti-Mouse IgG(H+L)(Elab Fluor® 488 conjugated)

一键复制产品信息

货号：E-AB-1056
文献（15）
说明书

说明书

Goat Anti-Mouse IgG(H+L)(Elab Fluor® 488 conjugated)

Price: ¥840.00 ¥210.00 ¥135.00 ¥80.00 ¥1470.00

Size:

500 μL 120 μL 60 μL 30 μL 1 mL

宿主： Goat
应用性： IF

Source	Goat
Immunogen	Mouse IgG
Application	IF
Recommended Dilution	IF 1:100-300
Concentration	0.5 mg/mL
Clonality	Polyclonal
Purification	Affinity purification
Conjugation	Elab Fluor®488
Amax	493nm
Emax	519nm
Storage Instructions	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Storage Buffer	PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4

DSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts

IF: 14.700

Journal： Nature Communications (2025)

DOI：10.1038/s41467-024-55063-7
The effects of autophagy-modifying drugs chloroquine and lithium on the skin melanoma microenvironment

IF: 2.700

Journal： TISSUE & CELL (2025)

DOI：10.1016/j.tice.2025.102753

Reactivity：Mouse

Sample Type：B16 cell-Xenograft
Acid-sensing ion channel-1 contributes to the failure of myelin sheath regeneration following spinal cord injury by transcellular delivery of PGE2

IF: 9.200

Journal： CELLULAR & MOLECULAR BIOLOGY LETTERS (2024)

DOI：10.1186/s11658-024-00672-9

Reactivity：Mouse,Rat

Sample Type：spinal cord tissue
Effect of opioid receptor antagonist on mitigating tumor necrosis factor-like weak inducer of apoptosis (TWEAK)-induced apoptolysis in pemphigus pathogenesis

IF: 7.900

Journal： JOURNAL OF AUTOIMMUNITY (2024)

DOI：10.1016/j.jaut.2024.103307

Reactivity：Mouse,Human

Sample Type：perilesional epidermis tissue,lesional skin tissue
Heat stress upregulates arachidonic acid to trigger autophagy in sertoli cells via dysfunctional mitochondrial respiratory chain function

IF: 6.100

Journal： Journal of Translational Medicine (2024)

DOI：10.1186/s12967-024-05182-y

Reactivity：Pig
Protective effect of astaxanthin on ANCA-associated vasculitis

IF: 5.600

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)

DOI：10.1016/j.intimp.2024.111928

PMID：38537540

Reactivity：Human
Synergistic effect of collagen peptide LR-7 and taurine on the prevention of cardiovascular injury in high salt-induced hypertensive mice

IF: 4.800

Journal： Food Bioscience (2024)

DOI：10.1016/j.fbio.2024.104907

Reactivity：mouse

Sample Type：hear
AP-1/C-FOS and AP-1/FRA2 differentially regulate early and late adipogenic differentiation of mesenchymal stem cells

IF: 4.000

Journal： JOURNAL OF CELLULAR BIOCHEMISTRY (2024)

DOI：10.1002/jcb.30543

PMID：38440920

Reactivity：Human
Arachidonic acid impairs the function of the blood-testis barrier via triggering mitochondrial complex-ROS-P38 MAPK axis in hyperthermal Sertoli cells

IF: 6.800

Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2023)

DOI：10.1016/j.ecoenv.2023.114598

PMID：36774800

Reactivity：Pig

Sample Type：boar testes
Effects of spray cryotherapy on cough receptors and airway microenvironment in a canine model of chronic bronchitis

IF: 2.700

Journal： CRYOBIOLOGY (2023)

DOI：10.1016/j.cryobiol.2023.104569

PMID：37597598

Reactivity：Dog

Sample Type：airway tissue

Q1:该如何选择二抗？

二抗根据一抗的来源和客户的实验需求来选择。
先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。
标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。
ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色；
WB主要选择HRP标记的二抗，用ECL底物发光液显色；
IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色；
IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。