Goat Anti-Mouse IgG(H+L)(FITC conjugated)（E-AB-1015）|-Elabscience伊莱瑞特【官网】

Goat Anti-Mouse IgG(H+L)(FITC conjugated)

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货号：E-AB-1015
文献（28）
说明书

说明书

Goat Anti-Mouse IgG(H+L)(FITC conjugated)

Price: ¥840.00 ¥210.00 ¥135.00 ¥80.00 ¥1470.00

Size:

500 μL 120 μL 60 μL 30 μL 1 mL

宿主： Goat
应用性： IF

Source	Goat
Immunogen	Mouse IgG
Application	IF
Recommended Dilution	IF 1:20-100
Concentration	0.5mg/mL
Clonality	Polyclonal
Purification	Affinity purification
Conjugation	FITC
Amax	495nm
Emax	525nm
Storage Instructions	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Storage Buffer	PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4

A Dual-Responsive Fe?O?@ZIF-8 Nanoplatform Combining Magnetic Targeting and pH Sensitivity for Low Back Pain Therapy

IF: 13.000

Journal： Small (2025)

DOI：10.1002/smll.202410874

Reactivity：Rat

Sample Type：L5 lumbar vertebrae tissue,dorsal root ganglia (DRG) tissue
Efficacy and mechanisms of concentrated growth factor on facial nerve rehabilitation in a rabbit model

IF: 5.800

Journal： Biomaterials Science (2025)

DOI：10.1039/D4BM01454E

Reactivity：Rabbit

Sample Type：facial nerve tissue
Integrated single-cell RNA sequencing reveals the tumor heterogeneity and microenvironment landscape during liver metastasis in adenocarcinoma of esophagogastric junction

IF: 5.700

Journal： Frontiers in Immunology (2025)

DOI：10.3389/fimmu.2024.1484234

Reactivity：Human

Sample Type：Adenocarcinoma of the esophagogastric junction (AEGJ) tumor tissue,normal sample
MIF/CD74 axis in hepatic stellate cells mediates HBV-related liver fibrosis

IF: 4.800

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)

DOI：10.1016/j.intimp.2024.113929

Reactivity：Human
Engineered Bacteriophage-Based In Situ Vaccine Remodels a Tumor Microenvironment and Elicits Potent Antitumor Immunity

IF: 15.800

Journal： ACS Nano (2024)

DOI：10.1021/acsnano.4c00413

Reactivity：Mouse
Neurophilic peptide-reinforced dual-fiber-network bioactive hydrogels for spinal cord injury repair

IF: 13.300

Journal： CHEMICAL ENGINEERING JOURNAL (2024)

DOI：10.1016/j.cej.2024.155301

Reactivity：Rat,Mouse,Human
Bioactive Peptide Hydrogel Scaffold with High Fluidity, Thermosensitivity, and Neurotropism in 3D Spatial Structure for Promoted Repair of Spinal Cord Injury

IF: 13.000

Journal： Small (2024)

DOI：10.1002/smll.202406990

Reactivity：Rat

Sample Type：Spinal Cord tissue
Customized Vascular Repair Microenvironment: Poly(lactic acid)-Gelatin Nanofibrous Scaffold Decorated with bFGF and Ag@Fe3O4 Core–Shell Nanowires

IF: 8.300

Journal： ACS Applied Materials & Interfaces (2024)

DOI：10.1021/acsami.4c09269

Reactivity：Mouse
Multifunctional natamycin modified chondroitin sulfate eye drops with anti-inflammatory, antifungal and tissue repair functions possess therapeutic effects on fungal keratitis in mice.

IF: 7.700

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

DOI：10.1016/j.ijbiomac.2024.135290

Reactivity：Mouse

Sample Type：eyeballs corneal tissue
Construction of bFGF/heparin and Fe3O4 nanoparticles functionalized scaffolds aiming at vascular repair and magnetic resonance imaging monitoring

IF: 7.700

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

DOI：10.1016/j.ijbiomac.2024.138416

Reactivity：Mouse,Rat

Q1:该如何选择二抗？

二抗根据一抗的来源和客户的实验需求来选择。
先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。
标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。
ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色；
WB主要选择HRP标记的二抗，用ECL底物发光液显色；
IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色；
IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。