Goat Anti-Rabbit IgG（E-AB-1034）|-Elabscience伊莱瑞特【官网】

Goat Anti-Rabbit IgG

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货号：E-AB-1034
文献（43）
说明书

说明书

Price: ¥160.00 ¥105.00

Size:

120 μL 60 μL

宿主： Goat
应用性： In WB, IF,IP,FCM, etc., can be used as IgG controls

Source	Goat
Immunogen	Rabbit IgG
Application	In WB, IF,IP,FCM, etc., can be used as IgG controls
Recommended Dilution	Other When the antibody is labeled, for example, with HRP, FITC, BIOTIN, etc, BSA and NaN3 cannot be contained in reagents.
Concentration	1 mg/mL
Clonality	Polyclonal
Purification	Affinity purification
Conjugation	Unconjugated
Storage Instructions	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Storage Buffer	PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4

Gastrodin Targets the System Xc-/GPX4 Axis to Inhibit Abnormal Proliferation of Fibroblast-Like Synoviocytes and Improve Rheumatoid Arthritis

IF: 6.700

Journal： PHYTOMEDICINE (2025)

DOI：10.1016/j.phymed.2025.156493

Reactivity：Rat

Sample Type：synovial tissue
AFB1 exposure promotes SIV replication and lung damage via RIG-I- and p38-mediated RETREG1/FAM134B-dependent endoplasmic reticulum autophagy

IF: 6.200

Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2025)

DOI：10.1016/j.ecoenv.2025.117970

Reactivity：Porcine

Sample Type：lung tissue
Piperine inhibits fungal growth and protects against pyroptosis in Aspergillus fumigatus keratitis by regulating the mTOR/HIF-1α pathway

IF: 4.800

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)

DOI：10.1016/j.intimp.2025.114286

Reactivity：Mouse

Sample Type：cornea tissue
Elevated expression and reduced phosphorylation of uterine RIG-I protein in pathogenic preterm labor in mice

IF: 3.700

Journal： REPRODUCTION (2025)

DOI：10.1530/REP-24-0299

Reactivity：Mouse

Sample Type：uterine tissue
Novel insights into bexarotene's role in preventing cholestasis: mechanisms and implications

IF: 3.100

Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2025)

DOI：10.1007/s00210-025-03917-2

Reactivity：Rat

Sample Type：liver tissue
Ginkgetin delays the progression of osteoarthritis by inhibiting the NF-κB and MAPK signaling pathways

IF: 2.800

Journal： Journal of Orthopaedic Surgery and Research (2025)

DOI：10.1186/s13018-025-05525-5

Reactivity：Human,Rat

Sample Type：cartilage tissue
The Rotenone-Induced Sporadic Parkinsonism Model: Timeline of Motor and Non-Motor Features

IF: 2.700

Journal： EUROPEAN JOURNAL OF NEUROSCIENCE (2025)

DOI：10.1111/ejn.16669

Reactivity：Rat

Sample Type：striatal tissue,midbrain tissue
A Novel Drug Candidate for Sepsis Targeting Heparanase by Inhibiting Cytokine Storm

IF: 14.300

Journal： Advanced Science (2024)

DOI：10.1002/advs.202403337

Reactivity：Mouse

Sample Type：Lung,kidney,spleens tissue
?hCeO2@ Cu5.4O nanoparticle alleviates inflammatory responses by regulating the CTSB-NLRP3 signaling pathway

IF: 7.300

Journal： Frontiers in Immunology (2024)

DOI：10.3389/fimmu.2024.1344098

PMID：38711511

Reactivity：Mouse
Licochalcone A Ameliorates Aspergillus fumigatus Keratitis by Reducing Fungal Load and Activating the Nrf2/HO-1 Signaling Pathway

IF: 4.000

Journal： ACS Infectious Diseases (2024)

DOI：10.1021/acsinfecdis.4c00123

Reactivity：Human

Q1:该如何选择二抗？

二抗根据一抗的来源和客户的实验需求来选择。
先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。
标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。
ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色；
WB主要选择HRP标记的二抗，用ECL底物发光液显色；
IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色；
IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。