Goat Anti-Rabbit IgG F(ab')2(peroxidase/HRP conjugated)（E-AB-1102）|-Elabscience伊莱瑞特【官网】

Goat Anti-Rabbit IgG F(ab')2(peroxidase/HRP conjugated)

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货号：E-AB-1102
文献（36）
说明书

说明书

Goat Anti-Rabbit IgG F(ab')2(peroxidase/HRP conjugated)

Price: ¥640.00 ¥160.00 ¥105.00 ¥70.00 ¥1200.00

Size:

500 μL 120 μL 60 μL 30 μL 1 mL

宿主： Goat
应用性： WB;IHC;ELISA

Source	Goat
Immunogen	Rabbit IgG
Application	WB, IHC, ELISA
Recommended Dilution	WB 1:8000-15000 (Enhanced chemiluminescent detection) WB 1:1000-5000(DAB detection) Direct ELISA 1:4000-8000(TMB detection) IHC 1:400-4000(DAB detection)
Concentration	0.3 mg/mL
Clonality	Polyclonal
Purification	Affinity purification
Conjugation	HRP
Storage Instructions	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Storage Buffer	PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4

S100P is a ferroptosis suppressor to facilitate hepatocellular carcinoma development by rewiring lipid metabolism

IF: 14.700

Journal： Nature Communications (2025)

DOI：10.1038/s41467-024-55785-8

Reactivity：Human

Sample Type：Hepatocellular carcinoma tissue
hCeO2@CA-074Me Nanoparticles Alleviate Inflammation and Improve Osteogenic Microenvironment by Regulating the CTSB-NLRP3 Signaling Pathway

IF: 6.600

Journal： International Journal of Nanomedicine (2025)

DOI：10.2147/IJN.S389156

Reactivity：Mouse
SLAMF8 Disrupts Epithelial Barrier in Chronic Rhinosinusitis with Nasal Polyps via M1 Macrophage Polarization

IF: 5.800

Journal： ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY (2025)

DOI：10.1016/j.anai.2025.01.020

Reactivity：Human
G3BP1 Regulates the Cell Cycle by Promoting IFNβ Production to Promote PCV2 Replication and Promotes Nuclear Transfer of Viral Proteins by Direct Binding

IF: 4.900

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2025)

DOI：10.3390/ijms26031083

Reactivity：Pig
Protective effects of olive oil antioxidant phenols on mercury-induced phosphatidylserine externalization in erythrocyte membrane: Insights into scramblase and flippase activity

IF: 7.100

Journal： FREE RADICAL BIOLOGY AND MEDICINE (2024)

DOI：10.1016/j.freeradbiomed.2024.11.047

Reactivity：Human
Long-Term Exposure of Fresh and Aged Nano Zinc Oxide Promotes Hepatocellular Carcinoma Malignancy by Up-Regulating Claudin-2

IF: 6.600

Journal： International Journal of Nanomedicine (2024)

DOI：10.2147/IJN.S478279

Reactivity：Human
Salusin?α alleviates lipid metabolism disorders via regulation of the downstream lipogenesis genes through the LKB1/AMPK pathway

IF: 5.700

Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2024)

DOI：10.3892/ijmm.2024.5397

Reactivity：Human
SMAR1 and p53-regulated lncRNA RP11-431M3.1 enhances HIF1A translation via miR-138 in colorectal cancer cells under oxidative stress

IF: 5.500

Journal： FEBS Journal (2024)

DOI：10.1111/febs.17253

Reactivity：Human
Effect of CFTR Modulators on Oxidative Stress and Autophagy in Non-CFTR-Expressing Cells

IF: 4.900

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)

DOI：10.3390/ijms251910360

Reactivity：Human
Possible role of apelin on the ovarian steroidogenesis and uterine apoptosis of infantile mice: An in vitro study

IF: 4.100

Journal： JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY (2024)

DOI：10.1016/j.jsbmb.2024.106463

PMID：38246202

Reactivity：Mouse

Sample Type：infantile ovary

Q1:该如何选择二抗？

二抗根据一抗的来源和客户的实验需求来选择。
先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。
标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。
ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色；
WB主要选择HRP标记的二抗，用ECL底物发光液显色；
IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色；
IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。