Goat Anti-Rabbit IgG F(ab')2(peroxidase/HRP conjugated)（E-AB-1102）|-Elabscience伊莱瑞特【官网】

Goat Anti-Rabbit IgG F(ab')2(peroxidase/HRP conjugated)

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货号：E-AB-1102
文献（42）
说明书

说明书

Goat Anti-Rabbit IgG F(ab')2(peroxidase/HRP conjugated)

Price: ¥640.00 ¥160.00 ¥105.00 ¥70.00 ¥1200.00

Size:

500 μL 120 μL 60 μL 30 μL 1 mL

宿主： Goat
应用性： WB;IHC;ELISA

Source	Goat
Immunogen	Rabbit IgG
Application	WB, IHC, ELISA
Recommended Dilution	WB 1:8000-15000 (Enhanced chemiluminescent detection) WB 1:1000-5000(DAB detection) Direct ELISA 1:4000-8000(TMB detection) IHC 1:400-4000(DAB detection)
Concentration	0.3 mg/mL
Clonality	Polyclonal
Purification	Affinity purification
Conjugation	HRP
Storage Instructions	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Storage Buffer	PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4

S100P is a ferroptosis suppressor to facilitate hepatocellular carcinoma development by rewiring lipid metabolism

IF: 14.7

Journal： Nature Communications (2025)

DOI：10.1038/s41467-024-55785-8

Reactivity：Human

Sample Type：Hepatocellular carcinoma tissue
hCeO2@CA-074Me Nanoparticles Alleviate Inflammation and Improve Osteogenic Microenvironment by Regulating the CTSB-NLRP3 Signaling Pathway

IF: 6.6

Journal： International Journal of Nanomedicine (2025)

DOI：10.2147/IJN.S389156

Reactivity：Mouse
SLAMF8 Disrupts Epithelial Barrier in Chronic Rhinosinusitis with Nasal Polyps via M1 Macrophage Polarization

IF: 5.8

Journal： ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY (2025)

DOI：10.1016/j.anai.2025.01.020

Reactivity：Human
G3BP1 Regulates the Cell Cycle by Promoting IFNβ Production to Promote PCV2 Replication and Promotes Nuclear Transfer of Viral Proteins by Direct Binding

IF: 4.9

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2025)

DOI：10.3390/ijms26031083

Reactivity：Pig
Study on the effect of deer bone in improving rheumatoid arthritis based on the “drug-target-pathway” association network

IF: 4.8

Journal： JOURNAL OF ETHNOPHARMACOLOGY (2025)

DOI：10.1016/j.jep.2025.119684

Reactivity：Human
Chimeric virus-like particles of nodavirus displaying M2e of human and avian influenza A viruses as a potential dual-use vaccine: Inducing a broader immune response and protecting mice against viral infections

IF: 4.5

Journal： VACCINE (2025)

DOI：10.1016/j.vaccine.2025.127165

Reactivity：Escherichia coli
Oroxylin A alleviates pyroptosis and apoptosis in human corneal epithelial cells under hyperosmotic stress by activating the SIRT3-SOD2/HIF-1α pathway

IF: 3.0

Journal： EXPERIMENTAL EYE RESEARCH (2025)

DOI：10.1016/j.exer.2025.110345

Reactivity：Human
Rutin resists Aspergillus fumigatus keratitis by activating Nrf2/HO-1 pathway, inhibiting Dectin-1/p-Syk pathway and affecting fungal structures

IF: 3.0

Journal： EXPERIMENTAL EYE RESEARCH (2025)

DOI：10.1016/j.exer.2025.110323

Reactivity：Mouse

Sample Type：cornea tissue
The therapeutic effect and mechanism of carnosic acid in Aspergillus fumigatus keratitis

IF: 3.0

Journal： EXPERIMENTAL EYE RESEARCH (2025)

DOI：10.1016/j.exer.2025.110338

Reactivity：Mouse

Sample Type：corneas tissue
EGR3 deficiency alleviates cigarette smoke-induced pulmonary inflammation in COPD through TLR4/NF-κB/TIMP-1 axis

IF: 2.5

Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2025)

DOI：10.1016/j.bbrc.2025.151741

Reactivity：Mouse

Sample Type：lung tissue

Q1:该如何选择二抗？

二抗根据一抗的来源和客户的实验需求来选择。
先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。
标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。
ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色；
WB主要选择HRP标记的二抗，用ECL底物发光液显色；
IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色；
IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。

Q2:有豚鼠抗酵母工程菌蛋白质抗体和兔抗酵母工程菌蛋白质抗体吗？你这里有抗猪IgG1，和抗猪IgG2a的二抗吗？

抗猪的二抗以及针对酵母工程菌的抗体，我们没有。