Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 647 conjugated)
Price:
- 宿主： Goat
- 应用性： IF
| Source | Goat |
| Immunogen | Rabbit IgG |
| Application | IF |
| Recommended Dilution | IF 1:100-300 |
| Concentration | 0.5 mg/mL |
| Clonality | Polyclonal |
| Purification | Affinity purification |
| Conjugation | Elab Fluor®647 |
| Amax | 650nm |
| Emax | 665nm |
| Storage Instructions | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
| Storage Buffer | PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4 |
-
A Dual-Responsive Fe?O?@ZIF-8 Nanoplatform Combining Magnetic Targeting and pH Sensitivity for Low Back Pain Therapy
IF: 13.000Journal： Small (2025)Reactivity：RatSample Type：L5 lumbar vertebrae tissue,dorsal root ganglia (DRG) tissue -
Viral IL-10 can promote the proliferation, migration and invasion of nasopharyngeal carcinoma cells and inhibit their apoptosis
IF: 2.800 -
Prenatal Hypoxia Triggers a Glucocorticoid-Associated Depressive-like Phenotype in Adult Rats, Accompanied by Reduced Anxiety in Response to Stress
IF: 4.900Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity：RatSample Type：hippocampus (HPC), prefrontal cortex (PFC), amygdala (AMG), and hypothalamus (HT) tissue -
Tumor-intrinsic IFNα and CXCL10 are critical for immunotherapeutic efficacy by recruiting and activating T lymphocytes in tumor microenvironment
IF: 4.600 -
The prenatal hypoxic pathology associated with maternal stress predisposes to dysregulated expression of the chrna7 gene and the subsequent development of nicotine addiction in adult offspring
IF: 4.100 -
AP-1/C-FOS and AP-1/FRA2 differentially regulate early and late adipogenic differentiation of mesenchymal stem cells
IF: 4.000 -
Licochalcone A Ameliorates Aspergillus fumigatus Keratitis by Reducing Fungal Load and Activating the Nrf2/HO-1 Signaling Pathway
IF: 4.000 -
Hybrid Structures Based on Silver Nanoparticles and Polyelectrolytes for Sensitive Fluorescent Solid-Phase Immunoassay
IF: 0.800Journal： Journal of Applied Spectroscopy (2024)Reactivity：Polystyrene plate -
PSTPIP2 regulates synovial macrophages polarization and dynamics via ERβ in the joint microenvironment
IF: 5.606Journal： ARTHRITIS RESEARCH & THERAPY (2022)PMID：36324152Reactivity：Human,MouseSample Type：synovial tissue
Q1:该如何选择二抗？
二抗根据一抗的来源和客户的实验需求来选择。
先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。
标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。
ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色；
WB主要选择HRP标记的二抗，用ECL底物发光液显色；
IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色；
IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。
