Goat Anti-Rabbit IgG(H+L)(FITC conjugated)（E-AB-1014）|-Elabscience伊莱瑞特【官网】

Goat Anti-Rabbit IgG(H+L)(FITC conjugated)

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货号：E-AB-1014
文献（62）
说明书

说明书

Goat Anti-Rabbit IgG(H+L)(FITC conjugated)

Price: ¥840.00 ¥210.00 ¥135.00 ¥80.00 ¥1470.00

Size:

500 μL 120 μL 60 μL 30 μL 1 mL

宿主： Goat
应用性： IF

Source	Goat
Immunogen	Rabbit IgG
Application	IF
Recommended Dilution	IF 1:20-100
Concentration	0.5mg/mL
Clonality	Polyclonal
Purification	Affinity purification
Conjugation	FITC
Amax	495nm
Emax	525nm
Storage Instructions	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Storage Buffer	PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4

A Dual-Responsive Fe?O?@ZIF-8 Nanoplatform Combining Magnetic Targeting and pH Sensitivity for Low Back Pain Therapy

IF: 13.000

Journal： Small (2025)

DOI：10.1002/smll.202410874

Reactivity：Rat

Sample Type：L5 lumbar vertebrae tissue,dorsal root ganglia (DRG) tissue
A novel chitosan hydrogel nanocomposite, embedded with polydopamine-modified graphitic carbon nitride, for accelerating the healing process of infected wounds

IF: 7.700

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025)

DOI：10.1016/j.ijbiomac.2025.140900

Reactivity：Mouse

Sample Type：Wound tissue
GelMA@LNP/AST Promotes eNOS-Dependent Angiogenesis Through Autophagy Activation for the Treatment of Hind Limb Ischemia

IF: 6.600

Journal： International Journal of Nanomedicine (2025)

DOI：10.2147/IJN.S499478

Reactivity：Mouse

Sample Type：gastrocnemius muscle tissue
Enhanced MRSA-infected wound healing using tannic acid cross-linked carboxymethyl chitosan/polyglutamic acid hydrogel for carbazole Delivery

IF: 5.400

Journal： COLLOIDS AND SURFACES B-BIOINTERFACES (2025)

DOI：10.1016/j.colsurfb.2024.114490

Reactivity：Mouse

Sample Type：wound tissue
MIF/CD74 axis in hepatic stellate cells mediates HBV-related liver fibrosis

IF: 4.800

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)

DOI：10.1016/j.intimp.2024.113929

Reactivity：Human
Multicomponent biopolymer hydrogels based on polycaprolactone with the combination of nano silver and linalool for the healing of infectious wounds

IF: 4.800

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)

DOI：10.1016/j.intimp.2025.114075

Reactivity：Mouse

Sample Type：skin tissue
Discovery of novel A2AR antagonist via 3D-QSAR pharmacophore modeling: neuroprotective effects in 6-OHDA-induced SH-SY5Y cells and haloperidol-induced Parkinsonism in C57 bl/6 mice

IF: 3.900

Journal： MOLECULAR DIVERSITY (2025)

DOI：10.1007/s11030-025-11120-x

Reactivity：Human
Network pharmacology uncovers that secoisolariciresinol diglucoside ameliorate premature ovarian insufficiency via PI3K/Akt pathway

IF: 3.800

Journal： Scientific Reports (2025)

DOI：10.1038/s41598-024-83484-3

Reactivity：Human
Anti-tumor efficacy and safety of AEV01 in preclinical glioblastoma and hepatocellular carcinoma models

IF: 3.800

Journal： Scientific Reports (2025)

DOI：10.1038/s41598-025-89594-w

Reactivity：Human
Accelerative effect of Carbazole nanocubosomes on the healing of methicillin-resistant Staphylococcus aureus-infected full-thickness wounds

IF: 2.400

Journal： Journal of Tissue Viability (2025)

DOI：10.1016/j.jtv.2025.100869

Reactivity：Mouse

Sample Type：wound tissue

Q1:该如何选择二抗？

二抗根据一抗的来源和客户的实验需求来选择。
先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。
标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。
ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色；
WB主要选择HRP标记的二抗，用ECL底物发光液显色；
IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色；
IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。