Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)（E-AB-1003）|-Elabscience伊莱瑞特【官网】

Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)

一键复制产品信息

货号：E-AB-1003
文献（346）
说明书

说明书

Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)

Price: ¥640.00 ¥160.00 ¥105.00 ¥70.00 ¥1200.00

Size:

500 μL 120 μL 60 μL 30 μL 1 mL

宿主： Goat
应用性： WB;IHC;ELISA

Source	Goat
Immunogen	Rabbit IgG
Application	WB, IHC, ELISA
Recommended Dilution	WB 1:5000-50000(Enhanced chemiluminescent detection) WB 1:1000-5000(DAB detection) Direct ELISA 1:5000-30000(TMB detection) IHC 1:500-2000(DAB detection)
Concentration	0.5 mg/mL
Clonality	Polyclonal
Purification	Affinity purification
Conjugation	HRP
Storage Instructions	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Storage Buffer	PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4

S100P is a ferroptosis suppressor to facilitate hepatocellular carcinoma development by rewiring lipid metabolism

IF: 14.700

Journal： Nature Communications (2025)

DOI：10.1038/s41467-024-55785-8

Reactivity：Human

Sample Type：Hepatocellular carcinoma tissue
Gastrodin Targets the System Xc-/GPX4 Axis to Inhibit Abnormal Proliferation of Fibroblast-Like Synoviocytes and Improve Rheumatoid Arthritis

IF: 6.700

Journal： PHYTOMEDICINE (2025)

DOI：10.1016/j.phymed.2025.156493

Reactivity：Rat

Sample Type：synovial tissue
hCeO2@CA-074Me Nanoparticles Alleviate Inflammation and Improve Osteogenic Microenvironment by Regulating the CTSB-NLRP3 Signaling Pathway

IF: 6.600

Journal： International Journal of Nanomedicine (2025)

DOI：10.2147/IJN.S389156

Reactivity：Mouse
Male sex determination maintains proteostasis and extends lifespan of daf-18/PTEN deficient C. elegans

IF: 6.500

Journal： EMBO REPORTS (2025)

DOI：10.1038/s44319-025-00368-x

Reactivity：Caenorhabditis elegan
AFB1 exposure promotes SIV replication and lung damage via RIG-I- and p38-mediated RETREG1/FAM134B-dependent endoplasmic reticulum autophagy

IF: 6.200

Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2025)

DOI：10.1016/j.ecoenv.2025.117970

Reactivity：Porcine

Sample Type：lung tissue
Efficacy and mechanisms of concentrated growth factor on facial nerve rehabilitation in a rabbit model

IF: 5.800

Journal： Biomaterials Science (2025)

DOI：10.1039/D4BM01454E

Reactivity：Rabbit

Sample Type：facial nerve tissue
SLAMF8 Disrupts Epithelial Barrier in Chronic Rhinosinusitis with Nasal Polyps via M1 Macrophage Polarization

IF: 5.800

Journal： ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY (2025)

DOI：10.1016/j.anai.2025.01.020

Reactivity：Human
Inflammation induced PFKFB3-mediated glycolysis promoting myometrium contraction through the PI3K-Akt-mTOR pathway in preterm birth mice

IF: 5.000

Journal： AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY (2025)

DOI：10.1152/ajpcell.00704.2024

Reactivity：Mouse

Sample Type：uterine tissue
G3BP1 Regulates the Cell Cycle by Promoting IFNβ Production to Promote PCV2 Replication and Promotes Nuclear Transfer of Viral Proteins by Direct Binding

IF: 4.900

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2025)

DOI：10.3390/ijms26031083

Reactivity：Pig
Prdx5 regulates macrophage polarization by modulating the TLR4/NF-κB pathway to promote apoptosis in chronic prostatitis

IF: 4.800

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)

DOI：10.1016/j.intimp.2025.114332

Reactivity：Mouse

Sample Type：prostate tissue

Q1:该如何选择二抗？

二抗根据一抗的来源和客户的实验需求来选择。
先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。
标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。
ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色；
WB主要选择HRP标记的二抗，用ECL底物发光液显色；
IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色；
IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。