HRP-conjugated beta actin Monoclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： Human;Mouse;Rat
- 应用性： WB
| Verified Samples |
Verified Samples in WB: Raji, Jurkat, HL-60, U87-MG, Mouse lung, Rat lung, Raw264.7, C6 |
| Dilution | WB 1:2500-1:5000 |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Recombinant human Beta actin protein by E. Coli |
| Abbre | beta actin |
| Synonyms | A26C1A, ACTB, Actin beta, Actin cytoplasmic 1, Actx, b actin |
| Swissprot | |
| Calculated MW | 43 kDa |
| Observed MW | 43 kDa |
| Cellular Localization | Cytoplasm>cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Signal Transduction, Tags and Cell Markers, Isotype/Loading, Controls, Neuroscience, Biochemicals, Cancer, Cardiovascular, Cell Biology, Immunology, Metabolism, Epigenetics and Nuclear Signaling, Stem Cells |
| Clone No. | 3A9 |
| Conjugation | HRP |
| Background | Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility,structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells,and commonly used as a loading control for western blot and as a control for qPCR. |
Western blot with HRP-conjugated beta actin Monoclonal Antibody at dilution of 1:5000.lane 1:Raji whole cell lysate,lane 2:Jurkat whole cell lysate, lane 3:HL-60 whole cell lysate,lane 4:U87-MG whole cell lysate,lane 5:mouse lung whole cell lysate,lane 6:rat lung whole cell lysate,lane 7:Raw264.7 whole cell lysate,lane 8:C6 whole cell lysate
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