HRP-conjugated beta Tubulin Monoclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： Human;Mouse;Rat;Zebrafish
- 应用性： WB
| Verified Samples |
Verified Samples in WB: Raji, Jurkat, HL-60, U87-MG, Mouse lung, Raw264.7, C6, Zebrafish |
| Dilution | WB 1:2500-1:5000 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Immunogen | Recombinant human TUBB protein by E. Coli |
| Abbre | beta Tubulin |
| Synonyms | Beta 4 tubulin, Beta 5 tubulin, Beta1 tubulin, Class I beta tubulin, TUBB, TUBB 1, TUBB 2, TUBB 5, TUBB1, TUBB2, TUBB5, Tu, Tubulin beta 2 chain, Tubulin beta chain, Tubulin beta class I, beta Ib tubulin, tubulin beta 1 chain, tubulin beta 5 chain, tubulin beta polypeptide |
| Swissprot | |
| Calculated MW | 55 kDa |
| Observed MW | 55 kDa |
| Cellular Localization | Cytoplasm, Cytoskeleton, Microtubule. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Signal Transduction, Neuroscience, Tags and Cell Markers, Isotype/Loading Controls, Biochemicals, Cancer, Cardiovascular, Cell Biology, Developmental Biology, Epigenetics and Nuclear Signaling, Immunology, Metabolism, Microbiology, Stem Cells |
| Clone No. | 2E8-2 |
| Conjugation | HRP |
| Background | In eukaryotes there are six members of the tubulin superfamily. α- and β-tubulins polymerize into microtubules,a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes,including mitosis. Both α and β tubulins have a mass of around 50 kDa. Tubulin was long thought to be specific to eukaryotes. Beta-tubulin gene is commonly used as a loading control for western blot. |
Western blot with HRP-conjugated beta Tubulin Monoclonal Antibody at dilution of 1:5000.lane 1:Raji whole cell lysate,lane 2:.Jurkat whole cell lysate,lane 3: HL-60 whole cell lysate ,lane 4:U87-MG whole cell lysate,lane 5:mouse lung whole cell lysate,lane 6:Raw264.7 whole cell lysate, lane 7:C6 whole cell lysate,lane 8:zebrafish whole cell lysate ,lane 9:zebrafish whole cell lysate
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