HRP-conjugated Flag-Tag Monoclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： All
- 应用性： WB
| Verified Samples |
Verified Samples in WB: 293T cells |
| Dilution | WB 1:2500-1:5000 |
| Clonality | Recombinant |
| Isotype | IgG2a |
| Immunogen | DYKDDDDK tag fusion protein |
| Abbre | Flag Tag |
| Synonyms | DDDDK epitope tag, DDDDK epitope tag, DYKDDDDK epitope tag |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Tags and Cell Markers, Microbiology, Immunology, Neuroscience |
| Clone No. | 3H9 |
| Conjugation | HRP |
| Background | FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology,having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This antibody detects FLAG tag in most applications. |
Western blot with HRP-conjugated Flag-Tag Monoclonal Antibody at dilution of 1:5000. lane 1: 293T cells transfected with Flag tagged recombinant protein, lane 2: 293T whole cell lysate
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