HRP-conjugated GAPDH Monoclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： Human;Mouse
- 应用性： WB
| Verified Samples |
Verified Samples in WB: Hela, MCF-7, HepG2, Raji, Raw264.7, Mouse kinedy, Mouse brain |
| Dilution | WB 1:2500-1:5000 |
| Clonality | Monoclonal |
| Isotype | IgM |
| Immunogen | Recombinant human GAPDH protein expressed by E.coli |
| Abbre | GAPDH |
| Synonyms | BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, aging associated gene 9 protein, cb609, p38 component |
| Swissprot | |
| Calculated MW | 36 kDa |
| Observed MW | 36 kDa |
| Cellular Localization | Cytoplasm>cytosol, Nucleus, Cytoplasm>perinuclear region, Membrane, Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity), Postnuclear and Perinuclear regions. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
| Buffer | PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Signal Transduction, Metabolism, Cancer, Neuroscience, Isotype/Loading Controls, Cell Biology, Epigenetics and Nuclear Signaling, Tags and Cell Markers, Biochemicals, Cardiovascular, Developmental Biology |
| Clone No. | 7B8 |
| Conjugation | HRP |
| Background | GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells,it is commonly used as a loading control for western blot and as a control for qPCR. |
Western blot with HRP-conjugated GAPDH Monoclonal Antibody at dilution of 1:5000.lane 1:Hela whole cell lysate,lane 2:MCF-7 whole cell lysate,lane 3:HepG2 whole cell lysate, lane 4:Raji whole cell lysate,lane 5:Raw264.7 whole cell lysate,lane 6:Mouse kinedy,lane 7:Mouse brain
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