HRP-conjugated GAPDH Monoclonal Antibody（AN00296HP）|-Elabscience伊莱瑞特【官网】

HRP-conjugated GAPDH Monoclonal Antibody

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货号：AN00296HP
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说明书

说明书

HRP-conjugated GAPDH Monoclonal Antibody

Price: ¥920.00 ¥350.00

Size:

100 μL 25 μL

宿主： Mouse
反应性： Human;Mouse
应用性： WB

Verified Samples	Verified Samples in WB: Hela, MCF-7, HepG2, Raji, Raw264.7, Mouse kinedy, Mouse brain
Dilution	WB 1:2500-1:5000
Clonality	Monoclonal
Isotype	IgM
Immunogen	Recombinant human GAPDH protein expressed by E.coli
Abbre	GAPDH
Synonyms	BARS-38, G3PDH, GAPD, KNC-NDS6, OCAS, aging associated gene 9 protein, cb609, p38 component
Swissprot	P04406
Calculated MW	36 kDa
Observed MW	36 kDa
Cellular Localization	Cytoplasm>cytosol, Nucleus, Cytoplasm>perinuclear region, Membrane, Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity), Postnuclear and Perinuclear regions.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.
Buffer	PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
Purification Method	Protein A/G Purification
Research Areas	Signal Transduction, Metabolism, Cancer, Neuroscience, Isotype/Loading Controls, Cell Biology, Epigenetics and Nuclear Signaling, Tags and Cell Markers, Biochemicals, Cardiovascular, Developmental Biology
Clone No.	7B8
Conjugation	HRP
Background	GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells,it is commonly used as a loading control for western blot and as a control for qPCR.

Western blot with HRP-conjugated GAPDH Monoclonal Antibody at dilution of 1:5000.lane 1:Hela whole cell lysate,lane 2:MCF-7 whole cell lysate,lane 3:HepG2 whole cell lysate, lane 4:Raji whole cell lysate,lane 5:Raw264.7 whole cell lysate,lane 6:Mouse kinedy,lane 7:Mouse brain