HRP-conjugated His-Tag Monoclonal Antibody

Price:
Size:
100 μL
25 μL
- 宿主: Mouse
- 反应性: All
- 应用性: WB
Verified Samples |
Verified Samples in WB: Purified recombinant protein |
Dilution | WB 1:2500-1:5000 |
Clonality | Recombinant |
Isotype | IgG2a |
Immunogen | His tag fusion protein |
Abbre | His Tag |
Synonyms | HHHHHH epitope tag, HHHHHH tag, Hexa His tag, His tag, Polyhistidine Tag, 6 His epitope tag |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
Purification Method | Protein A/G Purification |
Research Areas | Tags and Cell Markers, Microbiology |
Clone No. | 1A5 |
Conjugation | HRP |
Background | The 6xHis tag, also known as polyhistidine tag, His6 tag and/or hexa histidine tag, is an amino acid motif consisting of at least 6 histidine residues fused to the carboxyl (C-) or amino (N-) terminus of a target protein in transfected cells. It is one of the simplest and most widely used purification tags in the world. |
Western blot with HRP-conjugated His-Tag Monoclonal Antibody at dilution of 1: 5000. lane 1: Purified recombinant protein 1(100ng), lane 2: Purified recombinant protein 1(50ng), lane 3: Purified recombinant protein 2(100ng), lane 4: Purified recombinant protein 1(50ng)
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Applied Research Note: Development and Validation of a Highly Specific Polyclonal Antibody Targeting Neuraminidase of Novel H3N8 Avian Influenza Virus
IF: 1.900Journal: JOURNAL OF APPLIED POULTRY RESEARCH (2024)Reactivity:Escherichia coli -
Expression of Influenza M2e-NP Recombinant Fusion Protein in Escherichia coli BL21 (DE3) and Its Binding to Antibodies
IF: 4.961