HRP-conjugated PCNA Monoclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： Human;Mouse;Rat
- 应用性： WB
| Verified Samples |
Verified Samples in WB: Raji,HepG2,HL-60,Raw264.7,NIH/3T3,PC-12 |
| Dilution | WB 1:2500-1:5000 |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Immunogen | Recombinant human PCNA protein expressed by E.coli |
| Abbre | PCNA |
| Synonyms | Proliferating cell nuclear antigen,PCNA,Cyclin |
| Swissprot | |
| Calculated MW | 29 kDa |
| Observed MW | 29 kDa |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Clone No. | 9C6 |
| Conjugation | HRP |
| Background | Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. |
Western blot with HRP-conjugated PCNA Monoclonal Antibody at dilution of 1:5000. lane 1: Raji whole cell lysate, lane 2: HepG2 whole cell lysate, lane 3: HL-60 whole cell lysate, lane 4: Raw264.7 whole cell lysate, lane 5: NIH/3T3 whole cell lysate, lane 6: PC-12 whole cell lysate
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