HRP-conjugated Vinculin Monoclonal Antibody
Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： Human;Mouse;Rat
- 应用性： WB
| Verified Samples |
Verified Samples in WB: Raji, HL-60, U87-MG, Mouse lung, Rat lung, C6 |
| Dilution | WB 1:2500-1:5000 |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Recombinant human Vinculin protein expressed by E.coli |
| Abbre | Vinculin |
| Synonyms | Metavinculin (MV), VCL, Vinculin |
| Swissprot | |
| Calculated MW | 124 kDa |
| Observed MW | 135 kDa |
| Cellular Localization | Cytoplasm>cytoskeleton. Cell junction>adherens junction. Cell membrane. Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Cardiovascular, Signal Transduction, Isotype/Loading Controls, Epigenetics and Nuclear Signaling |
| Clone No. | 4D4 |
| Conjugation | HRP |
| Background | Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa,1,066-amino-acid protein which is ubiquitously expressed. Its splice variant,metavinculin (124 kDa),is muscle-specific. |
Western blot with HRP-conjugated Vinculin Monoclonal Antibody at dilution of 1:5000.lane 1:Raji whole cell lysate, lane 2:HL-60 whole cell lysate, lane 3:U87-MG whole cell lysate, lane 4:mouse lung whole cell lysate, lane 5:rat lung whole cell lysate, lane 6:C6 whole cell lysate
