HRP-Donkey Anti-rabbit IgG(H+L) preadsorbed
Price:
- 宿主： Donkey
- 应用性： WB;IHC;ELISA
| Source | Donkey |
| Immunogen | rabbit IgG |
| Application | WB, IHC, ELISA |
| Recommended Dilution | WB 1:5000-1:10000 IHC 1:250-1:500 ELISA 1:5000-1:10000 |
| Concentration | 0.5 mg/mL |
| Clonality | Polyclonal |
| Purification | Affinity purification |
| Conjugation | HRP |
| Storage Instructions | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4 |
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Q1:该如何选择二抗？
二抗根据一抗的来源和客户的实验需求来选择。
先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。
标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。
ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色；
WB主要选择HRP标记的二抗，用ECL底物发光液显色；
IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色；
IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。
