HRP-Goat Anti-Mouse IgG(H+L) preadsorbed
Price:
- 宿主： Goat
- 应用性： WB;IHC;ELISA
| Source | Goat |
| Immunogen | Mouse IgG |
| Application | WB, IHC, ELISA |
| Recommended Dilution | WB 1:5000-1:10000 IHC 1:250-1:500 ELISA 1:5000-1:10000 |
| Concentration | 0.5 mg/mL |
| Clonality | Polyclonal |
| Purification | Affinity purification |
| Conjugation | HRP |
| Storage Instructions | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4 |
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IF: 14.7Journal： Nature Communications (2025)Reactivity：HumanSample Type：Hepatocellular carcinoma tissue -
FABP4 as a Mediator of Lipid Metabolism and Pregnant Uterine Dysfunction in Obesity
IF: 14.3Journal： Advanced Science (2025)Reactivity：MouseSample Type：muscle tissue -
Astragalus polysaccharide alleviates oxidative stress and senescence in chondrocytes in osteoarthritis via GCN2/ATF4/TXN axis
IF: 7.7Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2025)Reactivity：MouseSample Type：mouse long bone tissue -
An FGF2-Derived Short Peptide Attenuates Bleomycin-Induced Pulmonary Fibrosis by Inhibiting Collagen Deposition and Epithelial–Mesenchymal Transition via the FGFR/MAPK Signaling Pathway
IF: 4.9Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2025)Reactivity：MouseSample Type：Lung tissue -
G3BP1 Regulates the Cell Cycle by Promoting IFNβ Production to Promote PCV2 Replication and Promotes Nuclear Transfer of Viral Proteins by Direct Binding
IF: 4.9 -
Loss of ADAMTS5 promotes vascular calcification via versican/integrin β1/FAK signal
IF: 4.9Journal： ATHEROSCLEROSIS (2025)Reactivity：HumanSample Type：human arteries tissue -
IGF1R Promotes Th17/Treg Cell Development in Experimental Autoimmune Prostatitis
IF: 4.2Journal： Journal of Inflammation Research (2025)Reactivity：MouseSample Type：prostate tissue -
Ganoderma atrum polysaccharide interferes with TLR4 against PD-1 inhibitors-induced carditis via NF-κB-NLRP3 pathway driven by IRF1/VEGFA/14–3-3γ axis in Lewis lung carcinoma mice
IF: 3.8Journal： Journal of Functional Foods (2025)Reactivity：MouseSample Type：myocardial tissue -
LncRNA NEAT1 sponges miR-214-3p to promote osteoblast differentiation through regulating the PI3K/AKT/mTOR pathway in aortic valve calcification
IF: 3.8 -
Sodium chloride promotes macrophage pyroptosis and aggravates rheumatoid arthritis by activating SGK1 through GABA receptors Slc6a12
IF: 8.2Journal： International Journal of Biological Sciences (2024)Reactivity：Mouse
Q1:该如何选择二抗？
二抗根据一抗的来源和客户的实验需求来选择。
先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。
标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。
ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色；
WB主要选择HRP标记的二抗，用ECL底物发光液显色；
IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色；
IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。
Q2:有豚鼠抗酵母工程菌蛋白质抗体和兔抗酵母工程菌蛋白质抗体吗？你这里有抗猪IgG1，和抗猪IgG2a的二抗吗？
抗猪的二抗以及针对酵母工程菌的抗体，我们没有。
