HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed（E-AB-1194）|-Elabscience伊莱瑞特【官网】

HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed

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货号：E-AB-1194
文献（34）
说明书

说明书

HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed

Price: ¥1418.00 ¥360.00 ¥2700.00

Size:

500 μL 120 μL 1 mL

宿主： Goat
应用性： WB;IHC;ELISA

Source	Goat
Immunogen	Rabbit IgG
Application	WB, IHC, ELISA
Recommended Dilution	WB 1:5000-1:10000 IHC 1:250-1:500 ELISA 1:5000-1:10000
Concentration	0.5 mg/mL
Clonality	Polyclonal
Purification	Affinity purification
Conjugation	HRP
Storage Instructions	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Storage Buffer	PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4

S100P is a ferroptosis suppressor to facilitate hepatocellular carcinoma development by rewiring lipid metabolism

IF: 14.700

Journal： Nature Communications (2025)

DOI：10.1038/s41467-024-55785-8

Reactivity：Human

Sample Type：Hepatocellular carcinoma tissue
Gastrodin Targets the System Xc-/GPX4 Axis to Inhibit Abnormal Proliferation of Fibroblast-Like Synoviocytes and Improve Rheumatoid Arthritis

IF: 6.700

Journal： PHYTOMEDICINE (2025)

DOI：10.1016/j.phymed.2025.156493

Reactivity：Rat

Sample Type：synovial tissue
hCeO2@CA-074Me Nanoparticles Alleviate Inflammation and Improve Osteogenic Microenvironment by Regulating the CTSB-NLRP3 Signaling Pathway

IF: 6.600

Journal： International Journal of Nanomedicine (2025)

DOI：10.2147/IJN.S389156

Reactivity：Mouse
AFB1 exposure promotes SIV replication and lung damage via RIG-I- and p38-mediated RETREG1/FAM134B-dependent endoplasmic reticulum autophagy

IF: 6.200

Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2025)

DOI：10.1016/j.ecoenv.2025.117970

Reactivity：Porcine

Sample Type：lung tissue
SLAMF8 Disrupts Epithelial Barrier in Chronic Rhinosinusitis with Nasal Polyps via M1 Macrophage Polarization

IF: 5.800

Journal： ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY (2025)

DOI：10.1016/j.anai.2025.01.020

Reactivity：Human
G3BP1 Regulates the Cell Cycle by Promoting IFNβ Production to Promote PCV2 Replication and Promotes Nuclear Transfer of Viral Proteins by Direct Binding

IF: 4.900

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2025)

DOI：10.3390/ijms26031083

Reactivity：Pig
Piperine inhibits fungal growth and protects against pyroptosis in Aspergillus fumigatus keratitis by regulating the mTOR/HIF-1α pathway

IF: 4.800

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2025)

DOI：10.1016/j.intimp.2025.114286

Reactivity：Mouse

Sample Type：cornea tissue
Elevated expression and reduced phosphorylation of uterine RIG-I protein in pathogenic preterm labor in mice

IF: 3.700

Journal： REPRODUCTION (2025)

DOI：10.1530/REP-24-0299

Reactivity：Mouse

Sample Type：uterine tissue
Novel insights into bexarotene's role in preventing cholestasis: mechanisms and implications

IF: 3.100

Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2025)

DOI：10.1007/s00210-025-03917-2

Reactivity：Rat

Sample Type：liver tissue
Ginkgetin delays the progression of osteoarthritis by inhibiting the NF-κB and MAPK signaling pathways

IF: 2.800

Journal： Journal of Orthopaedic Surgery and Research (2025)

DOI：10.1186/s13018-025-05525-5

Reactivity：Human,Rat

Sample Type：cartilage tissue

Q1:该如何选择二抗？

二抗根据一抗的来源和客户的实验需求来选择。
先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。
标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。
ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色；
WB主要选择HRP标记的二抗，用ECL底物发光液显色；
IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色；
IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。