Hydrogen Sulfide (H2S) Colorimetric Assay Kit (Indirect Method)
中文名称:硫化氢(H2S)比色法测试盒(间接法)

Price:
- 样本类型: 血清(浆);动物组织
- 检测仪器及波长: 酶标仪(660-670 nm,最佳检测波长为665 nm)
- 研究领域: 其他
检测原理 |
H2S在Fe3+存在下,与显色剂反应形成稳定的亚甲基蓝,亚甲基蓝在665 nm处有最大吸收峰,通过测定其吸光值可计算H2S含量。
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指标简称 | H2S |
灵敏度 | 0.15 μmol/L |
检测范围 | 0.76-100 μmol/L |
精密度 | 批间差:9.9% | 批内差:3.1% |
样本量 | 100 μL |
检测时间 | 1 h 20 min |
检测仪器及波长 | 酶标仪(660-670 nm,最佳检测波长为665 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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A sustained H2S-releasing nanocellulose-based hydrogel with anti-inflammatory and antibacterial properties for promoting infected wound healing
IF: 10.7Journal: CARBOHYDRATE POLYMERS (2025)Reactivity:MouseSample Type:Wound tissue -
Hydrogen sulfide preserves the function of senescent endothelium through SIRT2 mediated inflammatory inhibition
IF: 4.9Journal: JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (2025)Reactivity:MouseSample Type:plasma -
The role of L-cysteine/H2S pathway in CaSRs-mediated relaxations in mouse bladder tissue
IF: 2Journal: PHYSIOLOGICAL RESEARCH (2025)DOI:PMID:40698662Reactivity:MouseSample Type:bladder tissue -
Impairment of Endogenous H2S Pathway due to Aging and Endothelium Denudation in Mouse Isolated Thoracic Aorta
IF: 1.9 -
Electrical Dissipation Factor Measurements of Droplet Impact-Derived Microgels with Different Topological Structures
IF: 27.4Journal: ADVANCED MATERIALS (2024)Reactivity:Magnetic toroidal microgel (MTM) -
Self-immolative poly(thiocarbamate) with localized H2S signal amplification for precise cancer imaging and therapy
IF: 14.7Journal: Nature Communications (2024)Reactivity:Human,MouseSample Type:MCF7/ADR cell-Xenograft -
Dextran-Based Antibacterial Hydrogel Dressings for Accelerating Infected Wound Healing by Reducing Inflammation Levels
IF: 10.0Journal: Advanced Healthcare Materials (2024) -
Mitochondria-targeted hydrogen sulfide donor reduces fatty liver and obesity in mice fed a high fat diet by inhibiting de novo lipogenesis and inflammation via mTOR/SREBP-1 and NF-κB signaling pathways
IF: 9.1Journal: PHARMACOLOGICAL RESEARCH (2024)Reactivity:MouseSample Type:liver tissue -
Protective role of hydrogen sulfide against diabetic cardiomyopathy by inhibiting pyroptosis and myocardial fibrosis
IF: 6.9Journal: BIOMEDICINE & PHARMACOTHERAPY (2024)Reactivity:RatSample Type:myocardial tissue,serum -
Mitochondria-targeted hydrogen sulfide donor reduces atherogenesis by changing macrophage phenotypes and increasing UCP1 expression in vascular smooth muscle cells
IF: 6.9Journal: BIOMEDICINE & PHARMACOTHERAPY (2024)Reactivity:MouseSample Type:plasma
Q1:试剂二的作用是什么?是除蛋白吗?
这个试剂盒中,试剂一是用来形成沉淀的(试剂一含有大量的Zn离子会使蛋白变性析出);试剂二是溶解除了ZnS的其他沉淀;试剂三除了显色还可以溶解沉淀;试剂四除去样本蛋白,蛋白质对显色有干扰;试剂五就是形成亚甲基蓝。
Q2:加入试剂五之后,反应体系是呈酸性吗?
试剂四和试剂五是酸性,试剂二在后续操作过程中已经被洗干净,所以加入试剂五之后,反应体系是呈酸性的。
Q3:为什么标准曲线操作表和样本操作表加入的试剂不一样?
标准品是H2S,不含有其它蛋白,但是样本中存在一些对检测有干扰的蛋白,因此样本需要用试剂二和试剂四进行处理,但标准品不需要,所以标曲和样本测定的操作存在差异。

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