Hydrogen Peroxide (H2O2) Colorimetric Assay Kit
中文名称：过氧化氢（H2O2）比色法测试盒
Price:
Size:
96 T (80 samples)
48 T (32 samples)
- 样本类型： 血清（浆）;尿液;动植物组织;细胞
- 检测仪器及波长： 酶标仪（402-407 nm，最佳检测波长为405 nm）
- 研究领域： 氧化应激;植物生理系列;铁死亡
该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证
根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮 计算
| 检测原理 |
H2O2与钼酸铵反应生成稳定的黄色络合物且在405 nm处有最大吸收，黄色络合物的颜色深浅与H2O2的浓度在一定范围内具有线性关系。故可以通过比色计算出H2O2的含量。
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| 指标简称 | H2O2 |
| 灵敏度 | 0.41 mmol/L |
| 检测范围 | 0.41-125 mmol/L |
| 精密度 | 批间差：3.6% | 批内差：3.2% |
| 样本量 | 15 μL |
| 检测时间 | 30 min |
| 检测仪器及波长 | 酶标仪（402-407 nm，最佳检测波长为405 nm） |
| 保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
| 有效期 | 6个月 |
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| E-BC-F001 | Hydrogen Peroxide (H2O2) Fluorometric Assay Kit | 96 T (80 samples) , 48 T (32 samples) |
Q1:按照说明书操作，检测样本为什么没看到颜色变化？
虽然代谢试剂盒是基于化学反应检测显色物质的吸光度，但并不是所有试剂盒的显色物质颜色都是特别明显的。有些指标在样本中的浓度比较低，最终呈现的颜色可能通过肉眼无法判断，这是正常的，但是通过仪器是可以检测到的。
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