Hydrogen Peroxide (H2O2) Colorimetric Assay Kit
中文名称:过氧化氢(H2O2)比色法测试盒

Price:
- 样本类型: 血清(浆);尿液;动植物组织;细胞
- 检测仪器及波长: 酶标仪(402-407 nm,最佳检测波长为405 nm)
- 研究领域: 氧化应激;植物生理系列;铁死亡
生化试剂盒数量计算器x
需测样本数:
单个样本测量孔数:
试剂盒测样本孔数:
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量:
检测原理 |
H2O2与钼酸铵反应生成稳定的黄色络合物且在405 nm处有最大吸收,黄色络合物的颜色深浅与H2O2的浓度在一定范围内具有线性关系。故可以通过比色计算出H2O2的含量。
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指标简称 | H2O2 |
灵敏度 | 0.41 mmol/L |
检测范围 | 0.41-125 mmol/L |
精密度 | 批间差:3.6% | 批内差:3.2% |
检测时间 | 30 min |
检测仪器及波长 | 酶标仪(402-407 nm,最佳检测波长为405 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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E-BC-F001 | Hydrogen Peroxide (H2O2) Fluorometric Assay Kit | 96 T (80 samples) , 48 T (32 samples) |
Q1:按照说明书操作,检测样本为什么没看到颜色变化?
虽然代谢试剂盒是基于化学反应检测显色物质的吸光度,但并不是所有试剂盒的显色物质颜色都是特别明显的。有些指标在样本中的浓度比较低,最终呈现的颜色可能通过肉眼无法判断,这是正常的,但是通过仪器是可以检测到的。