Inhibition and Production of Superoxide Anionic Colorimetric Assay Kit(WST-1 Method)
中文名称：抑制与产生超氧阴离子自由基比色法测试盒（WST-1法）
Price:
Size:
96 T (44 samples)
48 T (20 samples)
- 样本类型： 血清（浆）;尿液;细胞;细胞上清;动植物组织
- 检测仪器及波长： 酶标仪（440-460 nm，最佳检测波长为450 nm）
- 研究领域： 氧化应激;铁死亡
该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证
根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮 计算
| 检测原理 |
通过黄嘌呤及黄嘌呤氧化酶反应系统产生超氧阴离子自由基，WST-1（一种水溶性四唑盐）可以与产生的超氧化物阴离子反应产生水溶性的甲臜染料，当被测样本中含有超氧阴离子自由基抑制剂时，可抑制甲臜染料的形成，当被测样本中含有产生超氧阴离子自由基物质时，促进甲臜染料的形成，通过对WST-1产物的比色分析可计算出样本抑制与产生超氧阴离子活力单位。
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| 指标简称 | CYYLZ |
| 精密度 | 批间差：5.8% | 批内差：2.0% |
| 样本量 | 40 μL |
| 检测时间 | 35 min |
| 检测仪器及波长 | 酶标仪（440-460 nm，最佳检测波长为450 nm） |
| 保存条件 | 该产品可在 2-8°C/-20°C避光条件下保存6个月，具体试剂组分的保存条件请参考说明书。 |
| 有效期 | 6个月 |
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