L-Lactic Acid (LA) Colorimetric Assay Kit

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中文名称：L-乳酸（LA）比色法测试盒

货号：E-BC-K044-M
文献（105）
说明书

说明书

L-Lactic Acid (LA) Colorimetric Assay Kit

Price: ¥1100.00 ¥620.00

Size:

96 T (80 samples) 48 T (32 samples)

样本类型： 血清（浆）;组织;细胞;细胞上清
检测仪器及波长： 酶标仪（520-540 nm，最佳检测波长为530 nm）
研究领域： 糖脂代谢;能量代谢

为了简化计算过程，可下载该产品计算文件。如使用中有任何问题，请随时联系技术支持

该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M

说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证

根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮计算

检测原理	以氧化型辅酶Ⅰ（NAD⁺）为氢受体，乳酸脱氢酶（LDH）催化乳酸脱氢产生丙酮酸，使NAD⁺转化为还原型辅酶Ⅰ（NADH）。其中N-甲基吩嗪甲基硫酸盐（PMS）递氢使氯化硝基四氮唑蓝（NBT）还原为紫色呈色物，呈色物的吸光度在530 nm时与乳酸含量呈线性关系。
指标简称	LA
灵敏度	0.10 mmol/L
检测范围	0.12-7.0 mmol/L
精密度	批间差：3.5% \| 批内差：1.4%
样本量	5 μL
检测时间	30 min
检测仪器及波长	酶标仪（520-540 nm，最佳检测波长为530 nm）
保存条件	该产品可在 2-8°C避光条件下保存6个月。
有效期	6个月

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