L-Lactic Acid (LA) Colorimetric Assay Kit
中文名称:L-乳酸(LA)比色法测试盒

Price:
Size:
96 T (80 samples)
48 T (32 samples)
- 样本类型: 血清(浆);组织;细胞;细胞上清
- 检测仪器及波长: 酶标仪(520-540 nm,最佳检测波长为530 nm)
- 研究领域: 糖脂代谢;能量代谢
为了简化计算过程,可下载该产品计算文件。如使用中有任何问题,请随时联系技术支持
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
以氧化型辅酶Ⅰ(NAD+)为氢受体,乳酸脱氢酶(LDH)催化乳酸脱氢产生丙酮酸,使NAD+转化为还原型辅酶Ⅰ(NADH)。其中N-甲基吩嗪甲基硫酸盐(PMS)递氢使氯化硝基四氮唑蓝(NBT)还原为紫色呈色物,呈色物的吸光度在530 nm时与乳酸含量呈线性关系。
|
指标简称 | LA |
灵敏度 | 0.10 mmol/L |
检测范围 | 0.12-7.0 mmol/L |
精密度 | 批间差:3.5% | 批内差:1.4% |
样本量 | 5 μL |
检测时间 | 30 min |
检测仪器及波长 | 酶标仪(520-540 nm,最佳检测波长为530 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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