Lactate Dehydrogenase (LDH) Activity Assay Kit
中文名称:乳酸脱氢酶(LDH)比色法测试盒

Price:
Size:
96 T (40 samples)
- 样本类型: 血清(浆);胸水;组织;细胞
- 检测仪器及波长: 酶标仪(440-460 nm,最佳检测波长为450 nm)
- 研究领域: 肝肾功能;糖脂代谢;能量代谢
为了简化计算过程,可下载该产品计算文件。如使用中有任何问题,请随时联系技术支持
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
以辅酶I为递氢体,LDH催化乳酸产生丙酮酸,丙酮酸与2,4-二硝基苯肼作用生成丙酮酸二硝基苯腙,后者在碱性溶液中呈棕红色,颜色的深浅与丙酮酸的浓度呈正比,通过测定OD值,可计算LDH的活力。
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指标简称 | LDH |
灵敏度 | 6 U/L |
检测范围 | 6-500 U/L |
精密度 | 批间差:2.4% | 批内差:1.8% |
样本量 | 40 μL |
检测时间 | 45 min |
检测仪器及波长 | 酶标仪(440-460 nm,最佳检测波长为450 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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Q1:这个试剂盒可以检测细胞培养液样本吗?样本含酚红是否会对测值结果有干扰?
细胞培养液含有酚红会干扰检测结果,所以这个试剂盒不适用于检测细胞培养液。检测细胞培养液样本,推荐E-BC-K766-M试剂盒,该试剂盒的灵敏度更高,且不受酚红的干扰。
Q2:试剂盒反应体系的总体积是325μL,酶标板是否可以装的下,会不会溢出?
使用试剂盒中配套的酶标板,是可以装下不会溢出的。不同厂家的酶标板孔最大容积可能存在微小差异,若换用其他的酶标板,有可能出现溢出的情况。