Lactate Dehydrogenase (LDH) Activity Assay Kit
中文名称：乳酸脱氢酶（LDH）比色法测试盒
Price:
Size:
96 T (40 samples)
- 样本类型： 血清（浆）;胸水;组织;细胞
- 检测仪器及波长： 酶标仪（440-460 nm，最佳检测波长为450 nm）
- 研究领域： 肝肾功能;糖脂代谢;能量代谢
为了简化计算过程，可下载该产品计算文件。如使用中有任何问题，请随时联系技术支持
该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证
根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮 计算
| 检测原理 |
以辅酶I为递氢体，LDH催化乳酸产生丙酮酸，丙酮酸与2,4-二硝基苯肼作用生成丙酮酸二硝基苯腙，后者在碱性溶液中呈棕红色，颜色的深浅与丙酮酸的浓度呈正比，通过测定OD值，可计算LDH的活力。
|
| 指标简称 | LDH |
| 灵敏度 | 6 U/L |
| 检测范围 | 6-500 U/L |
| 精密度 | 批间差：2.4% | 批内差：1.8% |
| 样本量 | 40 μL |
| 检测时间 | 45 min |
| 检测仪器及波长 | 酶标仪（440-460 nm，最佳检测波长为450 nm） |
| 保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
| 有效期 | 6个月 |
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