M-CSF Polyclonal Antibody(Capture/Detector)
Price:
- 宿主： Rabbit
- 反应性： Rat
- 应用性： ELISA Capture/Detector
| Verified Samples |
Verified Samples in ELISA: Recombinant Rat M-CSF protein, Rat serum, Rat plasma |
| Dilution | ELISA Capture 2-8 μg/mL, ELISA Detector 0.1-0.4 μg/mL |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | Recombinant Rat M-CSF Protein expressed by E.coli |
| Abbre | M-CSF |
| Synonyms | Macrophage colony-stimulating factor, CSF-1, Macrophage colony-stimulating factor 1, MCSF, M-CSF, Csfm, Csf1 |
| Swissprot | |
| Concentration | 1 mg/mL |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Buffer | Phosphate buffered solution, pH 7.2, containing 0.05% proclin 300. |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Background | M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF protein is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF protein can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M‑CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. |
| PDER100137 | Recombinant Rat M-CSF Protein(Trx Tag) | 100 μg , 20 μg |
