Malondialdehyde (MDA) Colorimetric Assay Kit (Cell Samples)
中文名称:丙二醛(MDA)比色法测试盒(测细胞)

Price:
- 样本类型: 细胞
- 检测仪器及波长: 酶标仪(530-540 nm,最佳检测波长为532 nm)
- 研究领域: 氧化应激;铁死亡
检测原理 |
过氧化脂质降解产物中的丙二醛(MDA)在高温及酸性环境下可与硫代巴比妥酸(TBA) 反应产生红棕色产物3,5,5’-三甲基恶唑2,4-二酮(三甲川) ,该物质在532 nm处有最大吸收峰。
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指标简称 | MDA |
灵敏度 | 0.29 nmol/mL |
检测范围 | 0.29-100 nmol/mL |
精密度 | 批间差:3.5% | 批内差:3.3% |
样本量 | 100 μL(cell homogenate) |
检测时间 | 1 h 10 min |
检测仪器及波长 | 酶标仪(530-540 nm,最佳检测波长为532 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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Q1:试剂盒中的匀浆介质可以用生理盐水 (0.9% NaCl)或者PBS(0.01 M, pH 7.4)替代吗?
这个试剂盒的样本匀浆介质只能用说明书中要求的试剂五,不能用生理盐水 (0.9% NaCl)或者PBS(0.01 M, pH 7.4)替换。
Q2:试剂三打开是固体状态正常吗?这种情况能否继续使用呢?
试剂三存放后可能会有固体析出,是正常现象。在80℃加热溶解,冷却后可以正常使用。
Q3:检测细胞样本,匀浆后制成细胞悬液,如果暂时不测,最多能存放几天?
已经匀浆处理的细胞悬液建议当天检测,不能冻存。如果暂时不测,可以直接冻存细胞,一般建议-80℃不超过一个月。
Q4:样本经过试剂五处理后的上清可以用于WB实验吗?
可以。
Q5:MDA细胞检测的试剂盒,破碎细胞可以用超声清洗仪吗?
可以,一般建议超声时间3~5min

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