Malondialdehyde (MDA) Colorimetric Assay Kit (Cell Samples)
中文名称:丙二醛(MDA)比色法测试盒(测细胞)

Price:
Size:
96 T (92 samples)
48 T (44 samples)
- 样本类型: 细胞
- 检测仪器及波长: 酶标仪(530-540 nm,最佳检测波长为532 nm)
- 研究领域: 氧化应激;铁死亡
为了简化计算过程,可下载该产品计算文件。如使用中有任何问题,请随时联系技术支持
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
过氧化脂质降解产物中的丙二醛(MDA)在高温及酸性环境下可与硫代巴比妥酸(TBA) 反应产生红棕色产物3,5,5’-三甲基恶唑2,4-二酮(三甲川) ,该物质在532 nm处有最大吸收峰。
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指标简称 | MDA |
灵敏度 | 0.29 nmol/mL |
检测范围 | 0.29-100 nmol/mL |
精密度 | 批间差:3.5% | 批内差:3.3% |
样本量 | 100 μL(cell homogenate) |
检测时间 | 1 h 10 min |
检测仪器及波长 | 酶标仪(530-540 nm,最佳检测波长为532 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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