Mitochondrial Complex II Activity Assay Kit
中文名称:线粒体呼吸链复合物II比色法测试盒

Price:
Size:
96 T (94 samples)
48 T (46 samples)
- 样本类型: 动物组织
- 检测仪器及波长: 酶标仪(590-610 nm,最佳检测波长为600 nm)
- 研究领域: 线粒体功能;铜死亡;能量代谢
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
线粒体呼吸链复合物Ⅱ又称琥珀酸-辅酶Q还原酶,广泛存在于动物、植物、微生物和培养细胞的线粒体中,它催化三羧酸循环中的琥珀酸氧化成延胡索酸(富马酸) ,通过复合物中的基本单元结构比如硫铁蛋白等,使泛醌转化为还原形态。复合体Ⅱ的催化产物还原型辅酶Q可进一步还原 2,6-二氯吲哚酚,2,6-二氯吲哚酚在600 nm有特征吸收峰,通过检测2,6-二氯吲哚酚的减少速率来计算该酶活性。
|
灵敏度 | 0.54 U/L |
检测范围 | 0.54-17.66 U/L |
精密度 | 批间差:5% | 批内差:4% |
样本量 | 190 μL(tissue/cell homogenate) |
检测时间 | 30 min |
检测仪器及波长 | 酶标仪(590-610 nm,最佳检测波长为600 nm) |
保存条件 | 该产品可在 -20°C避光条件下保存6个月。 |
有效期 | 6个月 |
-
OXPHOS inhibition overcomes chemoresistance in triple negative breast cancer
IF: 10.7 -
Oral Sulforaphane Intervention Protects Against Diabetic Cardiomyopathy in db/db Mice: Focus on Cardiac Lipotoxicity and Substrate Metabolism
IF: 6 -
Exploring Azithromycin’s Neuroprotective Role in Traumatic Brain Injury: Insights into Cognitive and Motor Recovery and Neuroinflammatory Modulation
IF: 4.3Journal: Pharmaceuticals (2025)Reactivity:RatSample Type:brain tissue,cerebrospinal fluid (CSF),blood plasma -
Luteolin alleviates muscle atrophy, mitochondrial dysfunction and abnormal FNDC5 expression in high fat diet-induced obese rats and palmitic acid-treated C2C12 myotubes
IF: 4.8Journal: JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2024)Reactivity:Mouse -
Biotoxicity of paraquat to lung cells mediated by endoplasmic reticulum-mitochondria interaction
IF: 2.9Journal: JOURNAL OF MOLECULAR HISTOLOGY (2024)Reactivity:RatSample Type:lung tissue -
Electroacupuncture improves cognitive impairment after ischemic stroke based on regulation of mitochondrial dynamics through SIRT1/PGC-1α pathway
IF: 2.7Journal: BRAIN RESEARCH (2024)Reactivity:RatSample Type:hippocampal tissue -
Coenzyme Q10 ameliorates chemotherapy-induced cognitive impairment in mice: a preclinical study
IF: 2.6Journal: MOLECULAR BIOLOGY REPORTS (2024)Reactivity:MouseSample Type:brain tissue -
17β-Estradiol (E2) Upregulates the ERα/SIRT1/PGC-1α Signaling Pathway and Protects Mitochondrial Function to Prevent Bilateral Oophorectomy (OVX)-Induced Nonalcoholic Fatty Liver Disease (NAFLD)
IF: 7.0 -
Molecular mechanisms of AMPK/YAP/NLRP3 signaling pathway affecting the occurrence and development of ankylosing spondylitis
IF: 2.6
E-BC-K138-F | Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Green) | 96 T |
E-BC-K152-M | Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit | 96 T (94 samples) , 48 T (46 samples) |

暂无文件,请在搜索框输入识别码查询相关文件

名称 | 状态 | 有效期至 | 操作 | |
---|---|---|---|---|
{{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注:请在有效期内下载相关文件,过期后页面内将不提供文件下载及预览!
在线咨询