Mitochondrial Complex II Activity Assay Kit
中文名称:线粒体呼吸链复合物II比色法测试盒

Price:
Size:
96 T (94 samples)
48 T (46 samples)
- 样本类型: 动物组织
- 检测仪器及波长: 酶标仪(590-610 nm,最佳检测波长为600 nm)
- 研究领域: 线粒体功能;铜死亡;能量代谢
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
生化试剂盒数量计算器x
需测样本数:
单个样本测量孔数:
试剂盒测样本孔数:
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量:
检测原理 |
线粒体呼吸链复合物Ⅱ又称琥珀酸-辅酶Q还原酶,广泛存在于动物、植物、微生物和培养细胞的线粒体中,它催化三羧酸循环中的琥珀酸氧化成延胡索酸(富马酸) ,通过复合物中的基本单元结构比如硫铁蛋白等,使泛醌转化为还原形态。复合体Ⅱ的催化产物还原型辅酶Q可进一步还原 2,6-二氯吲哚酚,2,6-二氯吲哚酚在600 nm有特征吸收峰,通过检测2,6-二氯吲哚酚的减少速率来计算该酶活性。
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灵敏度 | 0.54 U/L |
检测范围 | 0.54-17.66 U/L |
精密度 | 批间差:0.05 | 批内差:0.04 |
检测时间 | 30 min |
检测仪器及波长 | 酶标仪(590-610 nm,最佳检测波长为600 nm) |
保存条件 | 该产品可在 -20°C避光条件下保存6个月。 |
有效期 | 6个月 |
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