Mouse Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)（E-AB-1058）|-Elabscience伊莱瑞特【官网】

Mouse Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)

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货号：E-AB-1058
文献（20）
说明书

说明书

Mouse Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)

Price: ¥945.00 ¥240.00 ¥145.00 ¥85.00 ¥1800.00

Size:

500 μL 120 μL 60 μL 30 μL 1 mL

宿主： Mouse
应用性： WB;ELISA

Source	Mouse
Immunogen	Rabbit IgG
Application	WB, ELISA
Recommended Dilution	WB 1:2000-5000(Enhanced chemiluminescent detection) WB 1:300-1500(DAB detection) Direct ELISA 1:3000-5000(TMB detection)
Concentration	0.5 mg/mL
Clonality	Polyclonal
Purification	Affinity purification
Conjugation	HRP
Storage Instructions	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Storage Buffer	PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4

SLAMF8 Disrupts Epithelial Barrier in Chronic Rhinosinusitis with Nasal Polyps via M1 Macrophage Polarization

IF: 5.800

Journal： ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY (2025)

DOI：10.1016/j.anai.2025.01.020

Reactivity：Human
Protective effects of olive oil antioxidant phenols on mercury-induced phosphatidylserine externalization in erythrocyte membrane: Insights into scramblase and flippase activity

IF: 7.100

Journal： FREE RADICAL BIOLOGY AND MEDICINE (2024)

DOI：10.1016/j.freeradbiomed.2024.11.047

Reactivity：Human
Long-Term Exposure of Fresh and Aged Nano Zinc Oxide Promotes Hepatocellular Carcinoma Malignancy by Up-Regulating Claudin-2

IF: 6.600

Journal： International Journal of Nanomedicine (2024)

DOI：10.2147/IJN.S478279

Reactivity：Human
SMAR1 and p53-regulated lncRNA RP11-431M3.1 enhances HIF1A translation via miR-138 in colorectal cancer cells under oxidative stress

IF: 5.500

Journal： FEBS Journal (2024)

DOI：10.1111/febs.17253

Reactivity：Human
Effect of CFTR Modulators on Oxidative Stress and Autophagy in Non-CFTR-Expressing Cells

IF: 4.900

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)

DOI：10.3390/ijms251910360

Reactivity：Human
Anti-Inflammatory Efficacy of Nanobody Specific to β-Glucan on a Fungal Cell Wall in a Murine Model of Fungal Keratitis

IF: 4.000

Journal： ACS Infectious Diseases (2024)

DOI：10.1021/acsinfecdis.4c00334

Reactivity：Mouse,Human

Sample Type：cornea tissue
Identification of key ferroptosis genes and mechanisms associated with breast cancer using bioinformatics, machine learning, and experimental validation

IF: 3.900

Journal： Aging-US (2024)

DOI：10.18632/aging.205459

Reactivity：Human
Role of Noninvasive Urinary Prothrombin Fragment 1 + 2 to Measure Blood Coagulation Indices and Dose of Acenocoumarol

IF: 3.800

Journal： JOURNAL OF PROTEOME RESEARCH (2024)

DOI：10.1021/acs.jproteome.4c00462

Reactivity：Human

Sample Type：urine tissue
Extracellular putrescine can augment the epithelial-mesenchymal transition of gastric cancer cells by promoting MAL2 expression by elevating H3K27ac in its promoter region

IF: 3.600

Journal： American Journal of Cancer Research (2024)

DOI：10.62347/BEUV4081

Reactivity：Human
Getting insights into chemical composition and antiherpetic capability of jujube (Ziziphus jujuba mill.) drupes

IF: 3.400

Journal： Heliyon (2024)

DOI：10.1016/j.heliyon.2024.e37037

Reactivity：Jujube (Zizyphus jujuba Mill)

Sample Type：peel fraction (Pe) tissue,pulp fraction (Pu) tissue,seed tissue

Q1:该如何选择二抗？

二抗根据一抗的来源和客户的实验需求来选择。
先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。
标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。
ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色；
WB主要选择HRP标记的二抗，用ECL底物发光液显色；
IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色；
IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。