Nitric Oxide (NO) Colorimetric Assay Kit
中文名称:一氧化氮(NO)比色法测试盒

Price:
Size:
96 T (80 samples)
48 T (32 samples)
- 样本类型: 血清(浆);动植物组织
- 检测仪器及波长: 酶标仪(540-550 nm)
- 研究领域: 氧化应激
为了简化计算过程,可下载该产品计算文件。如使用中有任何问题,请随时联系技术支持
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
NO在体内或水溶液中极易氧化生成NO2-,与显色剂生成淡红色偶氮化合物,生成偶氮化合物的浓度与NO的浓度具有线性关系,通过比色可以间接计算NO的浓度。试剂一和试剂二的作用,为除去样本有色物质的干扰。
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指标简称 | NO |
灵敏度 | 0.16 μmol/L |
检测范围 | 0.16-100 μmol/L |
精密度 | 批间差:3.7% | 批内差:2.4% |
样本量 | 200-300 μL |
检测时间 | 55 min |
检测仪器及波长 | 酶标仪(540-550 nm) |
保存条件 | 该产品可在 2-8°C/-20°C避光条件下保存6个月,具体试剂组分的保存条件请参考说明书。 |
有效期 | 6个月 |
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