Normal Goat Blocking Buffer
中文名称:浓缩型正常山羊血清

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- 应用性: IHC;IF
产品简介 | 本品为正常山羊血清,加入稳定剂,作为封闭液可效减少IHC/IF实验的非特异染色从而降低背景。 |
保存条件 | 2~8°C保存,有效期12个月。 |
注意事项 | 1.本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。 2.为了您的安全和健康,请穿实验服并戴一次性手套操作。 |
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E-IR-R110 | Normal Goat Blocking Buffer (Ready-to-Use) | 50 mL , 10 mL , 5 mL |
Q1:IHC实验,想知道抗原修复条件是什么?是用EDTA还是枸橼酸钾?
修复条件一般为高温高压或者微波加热修复。
例如:抗原修复(高压法) :压力锅中加入足以淹没切片的 10 mmol/ml 的枸橼酸盐缓冲液(pH 6.0) (配制:将抗原修复液溶于相应体积 ddH2O中,混匀),加热至沸腾,切片置耐热塑料切片架上,放入锅中,盖好锅盖,扣上压力阀,继续加热,设置保压 4 min,时间到后打开放气阀放气,压力归零后开锅盖将内锅取出放置室温冷却,待溶液冷却至室温后取出切片(约30 min)。
抗原修复(微波法) :烧杯中加入足以淹没切片的 10 mmol/ml 的枸橼酸盐缓冲液(pH 6.0) (配制:将抗原修复液溶于相应体积 ddH2O中,混匀),加热至沸腾,切片置耐热塑料切片架上,放入烧杯中,中小火加热30min后取出,放置室温冷却,待溶液冷却至室温后取出切片(约30 min)。
IHC修复液的选择建议参考抗体使用说明书,或者参考以下方案:人体样本,优先推荐使用pH9.0的EDTA修复液(临床样本);大/小鼠样本,优先推荐使用pH6.0的枸橼酸修复液(科研样本)。
Q2:IHC在数据表上是什么意思?是冷冻切片吗?与IHC-F区别?
IHC是免疫组化实验,使用的切片一般包含有IHC-P(石蜡切片)和IHC-F(冰冻切片)两种。