PCNA Monoclonal Antibody
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Price:
Size:
100 μL
25 μL
- 宿主： Mouse
- 反应性： Human;Mouse;Rat
- 应用性： WB;IP;IHC
| Verified Samples |
Verified Samples in WB: HT-29, Jurkat, THP-1, Mouse spleen, Raw264.7, C6, HeLa, NIH/3T3 Verified Samples in IP: THP-1 Verified Samples in IHC: Human lung cancer, Human ovary cancer |
| Dilution | WB 1:2000-1:4000, IP 4ug/sample, IHC 1:200-1:400 |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Immunogen | Recombinant human PCNA protein expressed by E.coli |
| Abbre | PCNA |
| Synonyms | Proliferating cell nuclear antigen,PCNA,Cyclin |
| Swissprot | |
| Calculated MW | 29 kDa |
| Observed MW | 33 kDa |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling, Tags & Cell Markers, Cancer, Isotype, Loading Controls |
| Clone No. | 9C6 |
| Conjugation | Unconjugated |
| Background | Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. |
Western blot with Anti PCNA Monoclonal Antibody at dilution of 1:3000. Lane 1: HT-29 cell lysate, Lane 2: Jurkat cell lysate, Lane 3: THP-1 cell lysate, Lane 4: Mouse spleen tissue lysate, Lane 5: Raw264.7 cell lysate, Lane 6: C6 cell lysate, Lane 7: HeLa cell lysate, Lane 8: NIH/3T3 cell lysate.
Immunoprecipitation analysis of 40ug extracts of THP-1 cell using 4μg PCNA Monoclonal Antibody. Western blot was performed from the immunoprecipitate using PCNA Monoclonal Antibody at a dilution of 1:1000. Lane 1: input, Lane 2: Mouse IgG Isotype Control, Lane 3: anti-PCNA Monoclonal antibody.
Immunohistochemistry of paraffin-embedded Human lung cancer using PCNA Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Human ovary cancer using PCNA Monoclonal Antibody at dilution of 1:200.
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Rosmanol Effectively Prevents Diethylnitrosamine-induced Hepatocellular Carcinoma in Rats by Regulating the PI3K/Akt Signaling Pathway
IF: 0.600Journal： Pharmacognosy Magazine (2024)Reactivity：RatSample Type：hepatic tissue
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