PE/Cyanine7 Anti-Mouse CD206/MMR Antibody[C068C2]（E-AB-F1135H）|-Elabscience伊莱瑞特【官网】

PE/Cyanine7 Anti-Mouse CD206/MMR Antibody[C068C2]

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货号：E-AB-F1135H
文献（20）
说明书

说明书

PE/Cyanine7 Anti-Mouse CD206/MMR Antibody[C068C2]

Price: ¥2220.00 ¥1380.00 ¥3540.00

Size:

100 Tests 50 Tests 100 Tests × 2

应用性： FCM;ICFCM
亚型： Rat IgG2a, κ
宿主： Rat

Alternate Names	MMR, MR, MRC1, macrophage mannose receptor, mannose receptor
Cellular Localization	Membrane;Endosome
Clone No	C068C2
Isotype	Rat IgG2a, κ
Leadtime	咨询
Background	CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.
Abbre	CD206
Swissprot	Q61830
Host	Rat
Reactivity	Mouse
Clonality	Monoclonal
Isotype Control	PE/Cyanine7 Rat IgG2a, κ Isotype Control[2A3]
Applications	FCM;ICFCM
Form	Liquid
Concentration	5 μL/Test
Conjugation	PE/Cyanine 7
Conjugation Information	PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).
Spectrum
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
Storage	This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
Expiration Date	12个月
Shipping	冰袋
Gene ID	17533

C57BL/6 murine abdominal macrophages elicited by starch broth are stained with FITC Anti-Mouse F4/80 Antibody and PE/Cyanine7 Anti-Mouse CD206 Antibody (Left). Abdominal macrophages are stained with FITC Anti-Mouse F4/80 Antibody and PE/Cyanine7 Rat IgG2a, κ Isotype Control (Right).

Cationic liposomes encapsulating IL-2 selectively induce apoptosis and significantly reduce the secretion of cytokines on M1-murine polarized macrophages

IF: 3.7

Journal： CYTOKINE (2025)

DOI：10.1016/j.cyto.2025.156903

Reactivity：Mouse
Chemotherapy-elicited extracellular vesicle CXCL1 from dying cells promotes triple-negative breast cancer metastasis by activating TAM/PD-L1 signaling

IF: 11.4

Journal： JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH (2024)

DOI：10.1186/s13046-024-03050-7

Reactivity：Mouse
Traditional medicine Xianglian pill suppresses high-fat diet-related colorectal cancer via inactivating TLR4/MyD88 by remodeling gut microbiota composition and bile acid metabolism

IF: 4.8

Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)

DOI：10.1016/j.jep.2024.118411

Reactivity：Mouse

Sample Type：Colon tissue
Zingerone enhances the antitumor activity of attenuated Salmonella-mediated cancer immunotherapy by promoting tumor infiltration by host immune cells

IF: 3.8

Journal： Journal of Functional Foods (2024)

DOI：10.1016/j.jff.2024.106017

Reactivity：Mouse
Metformin enhances the anti-tumor effects mediated by attenuated Salmonella typhimurium

IF: 3.8

Journal： Journal of Functional Foods (2024)

DOI：10.1016/j.jff.2024.106217

Reactivity：Mouse

Sample Type：CT26 cell-Xenograft
CpG-Conjugated Silver Nanoparticles as a Multifunctional Nanomedicine to Promote Macrophage Efferocytosis and Repolarization for Atherosclerosis Therapy

IF: 9.5

Journal： ACS Applied Materials & Interfaces (2023)

DOI：10.1021/acsami.3c11227

PMID：37910772

Reactivity：Mouse
Granulocyte-Macrophage Colony-Stimulating Factor Promotes Endometrial Repair after Injury by Regulating Macrophages in mice

IF: 3.4

Journal： JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2023)

DOI：10.1016/j.jri.2023.104156

PMID：37801891

Reactivity：Mouse

Sample Type：uterus
Non-cytotoxic Nanoparticles Re-educating Macrophages Achieving both Innate and Adaptive Immune Responses for Tumor Therapy

IF: 6.598

Journal： Asian Journal of Pharmaceutical Sciences (2022)

DOI：10.1016/j.ajps.2022.06.001

PMID：36101893

Reactivity：Mouse
IFN-γ enhances the antitumor activity of attenuated salmonella-mediated cancer immunotherapy by increasing M1 macrophage and CD4 and CD8 T cell counts and decreasing neutrophil counts

IF: 6.064

Journal： Frontiers in Bioengineering and Biotechnology (2022)

DOI：10.3389/fbioe.2022.996055

Reactivity：Mouse

Sample Type：CT26 cell xenografts
Polarized M2 macrophages induced by mechanical stretching modulate bone regeneration of the craniofacial suture for midfacial hypoplasia treatment

IF: 5.249

Journal： CELL AND TISSUE RESEARCH (2021)

DOI：10.1007/s00441-021-03533-5

PMID：34568957

Reactivity：Mouse

E-CK-A105	10×ACK Lysis Buffer	500 mL , 200 mL , 100 mL
E-CK-A107	Cell Staining Buffer	500 mL , 200 mL , 100 mL
E-CK-A109	Intracellular Fixation/Permeabilization Buffer Kit	500 Assays , 100 Assays , 50 Assays

Q11:IHC实验，想知道抗原修复条件是什么？是用EDTA还是枸橼酸钾？

修复条件一般为高温高压或者微波加热修复。
例如：抗原修复（高压法）：压力锅中加入足以淹没切片的 10 mmol/ml 的枸橼酸盐缓冲液（pH 6.0）（配制：将抗原修复液溶于相应体积 ddH2O中，混匀），加热至沸腾，切片置耐热塑料切片架上，放入锅中，盖好锅盖，扣上压力阀，继续加热，设置保压 4 min，时间到后打开放气阀放气，压力归零后开锅盖将内锅取出放置室温冷却，待溶液冷却至室温后取出切片（约30 min）。
抗原修复（微波法）：烧杯中加入足以淹没切片的 10 mmol/ml 的枸橼酸盐缓冲液（pH 6.0）（配制：将抗原修复液溶于相应体积 ddH2O中，混匀），加热至沸腾，切片置耐热塑料切片架上，放入烧杯中，中小火加热30min后取出，放置室温冷却，待溶液冷却至室温后取出切片（约30 min）。
IHC修复液的选择建议参考抗体使用说明书，或者参考以下方案：人体样本，优先推荐使用pH9.0的EDTA修复液（临床样本）；大/小鼠样本，优先推荐使用pH6.0的枸橼酸修复液（科研样本）。

Q12:IHC在数据表上是什么意思？是冷冻切片吗？与IHC-F区别？

IHC是免疫组化实验，使用的切片一般包含有IHC-P（石蜡切片）和IHC-F（冰冻切片）两种。