Purified Anti-Human CD45RO Antibody[UCHL1]
![Purified Anti-Human CD45RO Antibody[UCHL1]](http://files.elabscience.cn/assets/images/loading.png)
Price:
Size:
100 μg
25 μg
- 应用性： FCM
- 亚型： Mouse IgG2a, κ
- 宿主： Mouse
| Clone No | |
| Isotype | Mouse IgG2a, κ |
| Leadtime |
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| Verified Samples | Verified Samples in FCM: Human peripheral blood lymphocytes |
| Dilution |
FCM 2 μg/mL(1×105-5×105 cells)
|
| Background |
CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.
|
| Immunogen | Recombinant Human CD45RO protein |
| Abbre | CD45RO |
| Host | Mouse |
| Reactivity | Human |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Applications | |
| Research Areas | Cell Biology;Immunology;Inhibitory Molecules;Neuroscience;Neuroscience Cell Markers;Stem Cells |
| Concentration | ≥ 1 mg/mL |
| Conjugation | Unconjugated |
| Storage Buffer | PBS, pH 7.2. Contains 0.05% proclin 300. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Expiration Date | 12个月 |
| Shipping |
Ice bag |
Human peripheral blood lymphocytes were stained with 0.2 μg AF/LE Purified Anti-Human CD45RO Antibody[UCHL1](Right) and 0.2 μg Mouse IgG2a, κ Isotype Control(Left), followed by APC-conjugated Goat Anti-Mouse IgG Secondary Antibody, then anti-Human CD45RA FITC-conjugated Monoclonal Antibody and anti-Human CD3 PerCP-conjugated Monoclonal Antibody. Data shown were from CD3+ population.
| E-CK-A105 | 10×ACK Lysis Buffer | 500mL , 200mL , 100mL |
| E-CK-A107 | Cell Staining Buffer | 500mL , 200mL , 100mL |
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