Pyruvate Kinase (PK) Activity Assay Kit
中文名称:丙酮酸激酶(PK)比色法测试盒

Price:
Size:
96 T (96 samples)
48 T (48 samples)
- 样本类型: 血清(浆);动物组织;细胞
- 检测仪器及波长: 酶标仪(330-350 nm,最佳检测波长为340 nm)
- 研究领域: 糖脂代谢;三羧酸循环;能量代谢
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
在二磷酸腺苷(ADP)存在的条件下,丙酮酸激酶(PK)催化磷酸烯醇丙酮酸(PEP)转化成丙酮酸,丙酮酸被LDH转化为乳酸,同时NADH转化为NAD+,检测340 nm下吸光值变化来计算得到丙酮酸激酶的活力。
|
指标简称 | PK |
灵敏度 | 0.08 U/L |
检测范围 | 0.08-6.79 U/L |
精密度 | 批间差:0.07 | 批内差:0.05 |
样本量 | 10 μL |
检测时间 | 30 min |
检测仪器及波长 | 酶标仪(330-350 nm,最佳检测波长为340 nm) |
保存条件 | 该产品可在-20°C避光条件下保存6个月。 |
有效期 | 6个月 |
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