Rabbit Anti-Goat IgG(H+L)(peroxidase/HRP conjugated)（E-AB-1004）|-Elabscience伊莱瑞特【官网】

Rabbit Anti-Goat IgG(H+L)(peroxidase/HRP conjugated)

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货号：E-AB-1004
文献（5）
说明书

说明书

Rabbit Anti-Goat IgG(H+L)(peroxidase/HRP conjugated)

Price: ¥640.00 ¥160.00 ¥105.00 ¥70.00 ¥1200.00

Size:

500 μL 120 μL 60 μL 30 μL 1 mL

宿主： Rabbit
应用性： WB;IHC;ELISA

Source	Rabbit
Immunogen	Goat IgG
Application	WB, IHC, ELISA
Recommended Dilution	WB 1:8000-15000(Enhanced chemiluminescent detection) WB 1:1000-5000(DAB detection) Direct ELISA 1:4000-8000(TMB detection) IHC 1:400-4000(DAB detection)
Concentration	0.5 mg/mL
Clonality	Polyclonal
Purification	Affinity purification
Conjugation	HRP
Storage Instructions	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Storage Buffer	PBS with 0.1% PC300, 1% protective protein and 50% glycerol, pH 7.4

Atherosis-associated lnc_000048 activates PKR to enhance STAT1-mediated polarization of THP-1 macrophages to M1 phenotype

IF: 6.100

Journal： Neural Regeneration Research (2024)

DOI：10.4103/NRR.NRR-D-23-01355

PMID：38526285

Reactivity：Human
Dulaglutide provides protection against sepsis-induced lung injury in mice by inhibiting inflammation and apoptosis

IF: 5.000

Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2023)

DOI：10.1016/j.ejphar.2023.175730

PMID：37062504

Reactivity：Mouse

Sample Type：lung tissues
MRI contrast enhancement of liver pre-neoplasia using iron–tannic nanoparticles

IF: 3.119

Journal： RSC Advances (2020)

DOI：10.1039/D0RA07308C

PMID：35515681

Reactivity：Rat

Sample Type：Tissue Slice
The role of netrin-1 in the mouse cornea during Aspergillus fumigatus infection

IF: 3.361

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2019)

DOI：10.1016/j.intimp.2019.03.047

PMID：30952101

Reactivity：Mouse

Sample Type：Tissue homogenate,Cell lysate
The influence of nanotopography on organelle organization and communication

IF: 8.893

Journal： Nano Research (2016)

DOI：10.1007/s12274-016-1129-3

Reactivity：Human

Sample Type：Cell lysate

Q1:该如何选择二抗？

二抗根据一抗的来源和客户的实验需求来选择。
先看实验所用一抗的种属来源和亚型。根据一抗种属来源和亚型来决定二抗：如一抗是兔来源的IgG，那就选抗兔IgG的二抗，例如Goat Anti-Rabbit IgG；如一抗是小鼠来源的IgM，那就选抗小鼠IgM的二抗，例如Goat Anti-Mouse IgM。
标记物的选择。常见的标记物有有HRP、Biotin、荧光素等。
ELISA常用Biotin标记的二抗，后接一个链霉亲和素标记的HRP起到进一步信号放大作用，用TMB底物显色；
WB主要选择HRP标记的二抗，用ECL底物发光液显色；
IHC一般也是选择HRP标记的二抗，推荐使用免疫组化试剂盒（E-IR-R217或最新的E-IR-R220/221），一般用DAB底物液显色；
IF可按实验需要选择不同荧光素标记的二抗，如FITC、Cy3、PE等均可（尽量避开细胞的自发荧光），通过荧光显微镜检测荧光。