Recombinant GAPDH Monoclonal Antibody（AN004430L）|-Elabscience伊莱瑞特【官网】

Recombinant GAPDH Monoclonal Antibody

一键复制产品信息

货号：AN004430L
文献（27）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC;IF

Verified Samples	Verified Samples in WB: A431, Caco-2, Jurkat, K562, HeLa, PC-3, Ramos, TF-1, U20-S, HL-60, HT-29, Raji, HepG2, Mouse heart, Mouse liver, Mouse uterus, Mouse lung, Mouse kidney, Mouse brain, Mouse stomach, C6, PC-12, Rat heart, Rat liver, Rat spleen, Rat brain, Rat stomach, Rat testis, Rat thymus, Rat skeletal muscle Verified Samples in IHC: Human colon cancer, Human appendix, Human breast cancer, Human lung cancer, Rat ovary, Mouse ovary Verified Samples in IF: U-2 OS cells, NIH/3T3 cells, C6
Dilution	WB 1:20000-1:100000, IHC 1:2000-1:4000, IF 1:2000-1:4000
Clonality	Recombination
Isotype	IgG
Immunogen	Recombinant protein within Mouse GAPDH
Abbre	GAPDH
Synonyms	BARS-38, cb609, G3PDH, GAPD, KNC-NDS6, OCAS
Swissprot	P16858
Calculated MW	36 kDa
Observed MW	36 kDa
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% Proclin300, 1% protective protein and 50% glycerol, pH7.4
Purification Method	Protein A/G Purification
Research Areas	Isotype, Loading Controls, Signal Transduction, Cancer, Metabolism
Clone No.	20B8
Conjugation	Unconjugated
Background	GAPDH is an enzyme of 36kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells,it is commonly used as a loading control for western blot and as a control for qPCR.

Western Blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. Lane 1: A431 cell lysate,Lane 2: Caco-2 cell lysate, Lane 3: Jurkat cell lysate, Lane 4: K562 cell lysate, Lane 5: HeLa cell lysate, Lane 6: PC-3 cell lysate, Lane 7: Ramos cell lysate, Lane 8: TF-1 cell lysate, Lane 9: U20-S cell lysate, Lane 10: HL-60 cell lysate, Lane 11: HT-29 cell lysate, Lane 12: Raji cell lysate, Lane 13: HepG2 cell lysate

Western blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. lane 1: mouse heart tissue lysate, lane 2: mouse liver tissue lysate, lane 3: mouse uterus tissue lysate, lane 4: mouse lung tissue lysate, lane 5: mouse kidney tissue lysate, lane 6: mouse brain tissue lysate, lane 7: mouse stomach tissue lysate

Western Blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. Lane 1: C6 cell lysate, Lane 2: PC-12 cell lysate, Lane 3: Rat heart tissue lysate, Lane 4: Rat liver tissue lysate, Lane 5: Rat spleen tissue lysate, Lane 6: Rat brain tissue lysate, Lane 7:Rat stomach tissue lysate, Lane 8: Rat testis tissue lysate, Lane 9: Rat thymus tissue lysate, Lane 10: Rat skeletal muscle tissue lysate

Immunohistochemistry of paraffin-embedded human colon cancer using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.

Immunohistochemistry of paraffin-embedded rat ovary using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.

Immunohistochemistry of paraffin-embedded mouse ovary using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.

Immunofluorescence analysis of U-2 OS cells using Recombinant GAPDH Monoclonal Antibody at dilution of 1:1000

Immunofluorescence analysis of NIH/3T3 cells using Recombinant GAPDH Monoclonal Antibody at dilution of 1:1000

Immunofluorescence analysis of C6 using Recombinant GAPDH Monoclonal Antibody at dilution of 1:1000

Immunohistochemistry of paraffin-embedded human appendix using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.

Immunohistochemistry of paraffin-embedded human breast cancer using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.

Immunohistochemistry of paraffin-embedded human lung cancer using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.

Formononetin protects against Aspergillus fumigatus Keratitis: Targeting inflammation and fungal load

IF: 4.8

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)

DOI：10.1016/j.intimp.2024.112046

Reactivity：Mouse,Human
Synergistic effect of collagen peptide LR-7 and taurine on the prevention of cardiovascular injury in high salt-induced hypertensive mice

IF: 4.8

Journal： Food Bioscience (2024)

DOI：10.1016/j.fbio.2024.104907

Reactivity：mouse

Sample Type：hear
Near infrared-responsive quinacrine–gold hybrid nanoparticles deregulate HSP-70/P300-mediated H3K14 acetylation in ER/PR+ breast cancer stem cells

IF: 4.7

Journal： Nanomedicine (2024)

DOI：10.2217/nnm-2023-0269

Reactivity：人
Transdermal Delivery of Recombinant Human Growth Hormone by Liposomal Gel for Skin Photoaging Therapy

IF: 4.6

Journal： ACS Applied Bio Materials (2024)

DOI：10.1021/acsabm.4c00391

Reactivity：Human
Plumbagin inhibits fungal growth, HMGB1/LOX-1 pathway and inflammatory factors in A. fumigatus keratitis

IF: 4.0

Journal： Frontiers in Microbiology (2024)

DOI：10.3389/fmicb.2024.1383509

Reactivity：Mouse

Sample Type：cornea
Progranulin Protects against Aspergillus fumigatus Keratitis by Attenuating the Inflammatory Response through Enhancing Autophagy

IF: 4.0

Journal： ACS Infectious Diseases (2024)

DOI：10.1021/acsinfecdis.4c00236

Reactivity：Mouse

Sample Type：corneas tissue
LncRNA MIAT suppresses inflammation in LPS-induced J774A.1 macrophages by promoting autophagy through miR-30a-5p/SOCS1 axi

IF: 3.8

Journal： Scientific Reports (2024)

DOI：10.1038/s41598-024-73607-1

Reactivity：Mouse
CD38 modulates cytokine secretion by NK cells through the Sirt1/NF-κB pathway, suppressing immune surveillance in colorectal cancer

IF: 3.8

Journal： Scientific Reports (2024)

DOI：10.1038/s41598-024-79008-8

Reactivity：Human
Methylcellulose improves dissociation quality of adult human primary cardiomyocytes

IF: 3.4

Journal： Heliyon (2024)

DOI：10.1016/j.heliyon.2024.e31653

Reactivity：Human
Cathelicidin boosts the antifungal activity of neutrophils and improves prognosis during Aspergillus fumigatus keratitis

IF: 2.9

Journal： INFECTION AND IMMUNITY (2024)

DOI：10.1128/iai.00483-23

Reactivity：Human,Mouse

Sample Type：cornea tissue

暂无文件，请在搜索框输入识别码查询相关文件

名称	状态	有效期至	操作
{{item.文件名}}	{{item.status_str}}	{{item.timestamp_str}}	点击查看点击预览

*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！

技术资源

• 如何明智选择抗体？

• 二抗选择指南

• Western Blot内参抗体选择原则

• 免疫组化抗体选择要点

• Western实验步骤

• Western Blot常见问题分析

• 不同浓缩胶体积所对应的各种组分的取样量

• 石蜡切片免疫组化实验步骤

• 免疫荧光实验指南

• 免疫共沉淀实验指南

全部分类

Recombinant GAPDH Monoclonal Antibody

实验操作视频

技术资源