Recombinant Human ICOSL Protein(His Tag)（PDMH100324）|-Elabscience伊莱瑞特【官网】

Recombinant Human ICOSL Protein(His Tag)

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货号：PDMH100324
说明书

说明书

Recombinant Human ICOSL Protein(His Tag)

Price: ¥3600.00 ¥1200.00

Size:

100 μg 20 μg

表达系统： Mammalian
蛋白编码： O75144

别称	B7H;B7-H;B7 Homolog;B7-Like Protein Gl;B7-Related Protein;V-Set and Immunoglobulin Domain-Containing Protein;KIAA;VSIG;ICOSLG;B7-H2;B7H2;B7RP-1;B7RP1;CD275;GL50;ICOS-L;ICOSL;LICOS;B7 Homolog 2;B7-Like Protein Gl50;B7-Related Protein 1;ICOS Ligand;Protein Z39Ig;V-Set and Immunoglobulin Domain-Containing Protein 4;VSIG4;Z39IG;CRIg;KIAA0653; ICOSL; B7RP1; B7h;B7 H2;B7 homologue 2;B7 like protein Gl50;B7 related protein 1;B7RP 1;CD 275;CD275 antigen;GL 50;ICOS L;ICOS LG;Inducible T cell co stimulator ligand;Transmembrane protein B7 H2 ICOS ligand
表达系统	Mammalian
序列	Met1~Ser258
蛋白编码	O75144
种属	Human
计算分子量	28.3 kDa
表观分子量	40-60 kDa
标签	C-His
生物活性	Not validated for activity
纯度	> 90% as determined by reducing SDS-PAGE.
内毒素	< 1.0 EU/mg of the protein as determined by the LAL method
保存条件	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输条件	This product is provided as lyophilized powder which is shipped with ice packs.
制剂	Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
复溶方法	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
背景	Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. The present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production, and germinal center formation.

SDS-PAGE analysis of Human ICOSL proteins, 2 μg/lane of Recombinant Human ICOSL proteins was resolved with SDS-PAGE under reducing conditions, showing bands at 40-60 KD

Q1:重组蛋白有活性吗？可以做活性检测吗？

活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。

Q2:重组蛋白是否测序验证过？

在售重组蛋白默认不做测序分析/验证。

Q3:蛋白的运输条件是什么？

我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。

Q4:蛋白是通过什么方式纯化的？

一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。

Q5:蛋白可以用于细胞培养/注射小鼠吗？

内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。

Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？

默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。