Recombinant Human IL6RA/CD126 protein (His Tag)-Elabscience伊莱瑞特【官网】

Recombinant Human IL6RA/CD126 protein (His Tag)

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货号：PDMH100399
说明书

说明书

Recombinant Human IL6RA/CD126 protein (His Tag)

Price: ¥3600.00 ¥1200.00

Size:

100 μg 20 μg

表达系统： HEK293 Cells
蛋白编码： P08887

别称	Membrane glycoprotein;CD126;gp80;IL6Q;IL-6R;IL-6R 1;IL-6R subunit alpha;IL-6R-1;IL6RA;IL-6RA;IL-6R-Alpha;IL6RQ;Interleukin-6 receptor subunit alpha;Membrane glycoprotein 80;IL6R;IL-6R&alpha;alpha;CD 126;CD126 antigen;IL 6R;IL 6R 1;IL 6R alpha;IL-6 receptor alpha chain;IL-6 receptor subunit alpha;IL-6R 1 Membrane glycoprotein 80 (gp80);Interleukin 6 receptor;MGC30256
表达系统	HEK293 Cells
序列	Met1-Pro365
蛋白编码	P08887
种属	Human
计算分子量	40.0 kDa
表观分子量	65 kDa
标签	C-His
生物活性	Not validated for activity
纯度	> 85% as determined by reducing SDS-PAGE.
内毒素	< 1.0 EU/mg of the protein as determined by the LAL method
保存条件	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输条件	This product is provided as lyophilized powder which is shipped with ice packs.
制剂	Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
复溶方法	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
背景	Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.

SDS-PAGE analysis of Human IL6RA/CD126 proteins, 2μg/lane of Recombinant Human IL6RA/CD126 proteins was resolved with SDS-PAGE under reducing conditions, showing bands at 65 KD.

Q1:重组蛋白有活性吗？可以做活性检测吗？

活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。

Q2:重组蛋白是否测序验证过？

在售重组蛋白默认不做测序分析/验证。

Q3:蛋白的运输条件是什么？

我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。

Q4:蛋白是通过什么方式纯化的？

一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。

Q5:蛋白可以用于细胞培养/注射小鼠吗？

内毒素含量小于0.1 ng/μg (1 EU/μg)的蛋白都可以用于细胞培养/注射小鼠。

Q6:蛋白发货形式可以是冻干粉吗？可以发液体吗？

默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。