Recombinant Human IL-12 p35 protein(His Tag)
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- 表达系统： E.coli
- 蛋白编码： P29459
- 应用性： Cell culture
| 别称 |
NKSF;IL-12/IL-35 p;IL-12 subunit p;Interleukin 12/P;NK cell stimulatory factor chain;CLMF p;P35;CLMF;NFSK;NKSF1;IL-12A;CLMF p35;CLMF1;Cytotoxic Lymphocyte Maturation Factor 35 kDa;Cytotoxic lymphocyte maturation factor 35 kDa subunit;IL-12 subunit p35;IL-12/IL-35 p35;Interleukin-12 subunit alpha;NK cell stimulatory factor chain 1;IL12A;IL12B;Interleukin 12/P70;CLMF;IL-12A;NFSK;NKSF1
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| 表达系统 |
E.coli
|
| 序列 |
Arg 23-Phe 202
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| 蛋白编码 |
P29459
|
| 种属 |
Human
|
| 计算分子量 |
23.5 kDa
|
| 表观分子量 |
28 kDa
|
| 标签 |
C-His
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| 生物活性 |
Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBMC). The ED50 for this effect is <49 pg/mL.
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 0.1 EU per μg of the protein as determined by the LAL method.
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| 保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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| 运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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| 制剂 |
Lyophilized from sterile PBS,pH 8.0.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| 复溶方法 |
Please refer to the printed manual for detailed information.
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| 背景 |
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
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实验操作视频
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