S100A9 Polyclonal Antibody(Capture/Detector)
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- 宿主： Rabbit
- 反应性： Human
- 应用性： ELISA Capture/Detector
| Verified Samples |
Verified Samples in ELISA: Recombinant Human S100A9 protein, Human serum, Human plasma |
| Dilution | ELISA Capture 2-8 μg/mL, ELISA Detector 0.1-0.4 μg/mL |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | Recombinant Human S100A9 protein expressed by E.coli |
| Abbre | S100A9 |
| Synonyms | Protein S100-A9, CaLgranuLin-B,
CaLprotectin L1H subunit,
Leukocyte L1 compLex heavy chain, Migration inhibitory factor-reLated protein 14, MRP-14, p14, S100 caLcium-binding protein A9, S100A9, CAGB, CFAG, MRP14 |
| Swissprot | |
| Concentration | 1 mg/mL |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Buffer | Phosphate buffered solution, pH 7.2, containing 0.05% proclin 300. |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Immunology, Signal Transduction, Cancer, Cell Biology, Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Background | S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response.
It can induce neutrophil chemotaxis,adhesion,can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK,PI3K/AKT,and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism.
Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions.
The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism,modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase .
Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane,transferring arachidonic acid,an essential cofactor,to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX.
The extracellular functions involve pro-inflammatory,antimicrobial,oxidant-scavenging and apoptosis-inducing activities.
Its pro-inflammatory activity includes recruitment of leukocytes,promotion of cytokine and chemokine production,and regulation of leukocyte adhesion and migration .
Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TlR4) and receptor for advanced glycation endproducts (AGER).
Binding to TlR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the pro-inflammatory cascade.
Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth.
Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3 |
