Super Excellent Chemiluminescent Substrate (ECL)Detection Kit
中文名称：超敏化学发光 (ECL)底物液
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- 应用性： WB;ELISA
| 产品简介 | DIA·AN®超敏化学发光 (ECL) 底物液是一款辣根过氧化物酶 (HRP) 底物，可用于检测辣根过氧化物酶（HRP）标记的蛋白或核酸，用于蛋白质印迹或ELISA等分析蛋白含量，可兼容胶片和数字显影系统。
本产品所含特有成分能有效降低非特异发光和背景发光，并减缓信号衰减，使得30 min后信号值无明显减弱，120 min后发光强度仍能达到最高发光强度的约70%左右。 |
| 保存条件 | 2~8°C避光保存，保质期12个月。 |
| 注意事项 | 1. 曝光背景高，可能是膜没洗干净、二抗或一抗浓度过高、封闭液不合适导致，请做适当调整。 2. A液和B液在吸取过程中必须要更换吸头，A液和B液相互污染后会导致其逐渐失效。 3. 如无信号，可能是目的蛋白表达弱，可适当延长曝光时间。 4. 荧光迅速淬灭，可能是目的条带荧光信号过强，导致HRP快速消耗ECL底物，建议降低二抗浓度。 5. NaN3或金属螯合剂会抑制HRP 活性，溶液中应避免使用。 6. 工作液需现配现用。 |
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| E-IR-R307 | Excellent Chemiluminescent Substrate (ECL)Detection Kit | 500 mL , 100 mL , 50 mL |
Q1:ECL可以用于ELISA实验吗？
ECL可以用于ELISA实验，(ECL) 底物液是一款辣根过氧化物酶 (HRP) 底物，可用于检测辣根过氧化物酶（HRP）标记的蛋白，具体的用量可参考说明书操作。
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