Thiobarbituric Acid Reactants (TBARS) Fluorometric Assay Kit
中文名称:硫代巴比妥酸反应物(TBARS)荧光法测试盒

Price:
Size:
96 T (80 samples)
48 T (32 samples)
- 样本类型: 血清(浆);动物组织;细胞
- 检测仪器及波长: 荧光酶标仪(Ex/Em=520 nm/550 nm)
- 研究领域: 氧化应激
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
TBARS(Thiobarbituric acid reactive substances)主要指丙二醛,可以和TBA在高温和酸性条件下反应形成的粉红色的化合物,其浓度与样本中TBARS的浓度具有线性关系,通过测定激发波长530 nm,发射波长550 nm下荧光值可以间接计算TBARS的浓度。
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指标简称 | TBARS |
灵敏度 | 0.09 μmol/L |
检测范围 | 0.09-10 μmol/L |
精密度 | 批间差:2.8% | 批内差:1.7% |
样本量 | 0.1 mL |
检测时间 | 1 h 20 min |
检测仪器及波长 | 荧光酶标仪(Ex/Em=520 nm/550 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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