Total Oxidant Status (TOS) Colorimetric Assay Kit
中文名称:总氧化状态(TOS)比色法测试盒

Price:
Size:
96 T (80 samples)
48 T (32 samples)
- 样本类型: 血清(浆);组织;细胞
- 检测仪器及波长: 酶标仪(580-590 nm,最佳检测波长为590 nm)
- 研究领域: 氧化应激
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
检测原理 |
在酸性条件下,样本中的氧化性物质可将Fe2+氧化为Fe3+,后者与二甲酚橙高度结合产生一种蓝紫色的复合物。在溶液pH为2-3的范围内时,其最大吸收波长在590 nm附近,且颜色深浅程度在一定浓度、一定时间内与氧化物质的含量成正比,从而间接测定样本的总氧化能力。
|
指标简称 | TOS |
灵敏度 | 2.5 μmol H2O2 Equiv./L |
检测范围 | 2.5-100 μmol H2O2 Equiv./L |
精密度 | 批间差:3.5% | 批内差:2.3% |
样本量 | 20 μL |
检测时间 | 30 min |
检测仪器及波长 | 酶标仪(580-590 nm,最佳检测波长为590 nm) |
保存条件 | 该产品可在 2-8°C避光条件下保存3个月。 |
有效期 | 3个月 |
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E-BC-K138-F | Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Green) | 96 T |
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