Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

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中文名称：总超氧化物歧化酶（T-SOD）比色法测试盒（羟胺法）

货号：E-BC-K019-M
文献（254）
说明书

说明书

Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

Price: ¥750.00 ¥450.00

Size:

96 T (92 samples) 48 T (44 samples)

样本类型： 血清（浆）;尿液;动植物组织;细胞;细胞上清
检测仪器及波长： 酶标仪（540-560 nm，最佳检测波长为550 nm）
研究领域： 氧化应激;植物生理系列

为了简化计算过程，可下载该产品计算文件。如使用中有任何问题，请随时联系技术支持

该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M

说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证

根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮计算

检测原理	通过黄嘌呤及黄嘌呤氧化酶反应系统产生超氧阴离子自由基（O₂^-），它氧化羟胺形成亚硝酸盐，在显色剂作用下产生紫红色化合物。当反应体系中加入SOD后，催化超氧阴离子自由基发生歧化反应，使形成的亚硝酸盐减少，进而产生的紫红色变浅。根据样品管与对照管中的吸光度差值，来计算SOD的活性。
指标简称	T-SOD
灵敏度	2.4 U/mL
检测范围	2.4-61 U/mL
精密度	批间差：5.6% \| 批内差：5.5%
样本量	5 μL
检测时间	1 h 10 min
检测仪器及波长	酶标仪（540-560 nm，最佳检测波长为550 nm）
保存条件	该产品可在 2-8°C/-20°C避光条件下保存6个月，具体试剂组分的保存条件请参考说明书。
有效期	6个月

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E-BC-K020-M	Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)	96 T (92 samples) , 48 T (44 samples)
E-BC-K022-M	CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)	96 T (92 samples) , 48 T (44 samples)