Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)
中文名称:总超氧化物歧化酶(T-SOD)比色法测试盒(WST-1 法)

Price:
- 样本类型: 血清(浆);胸水;腹水;尿液;细胞;动植物组织
- 检测仪器及波长: 酶标仪(440-460 nm)
- 研究领域: 氧化应激;植物生理系列;铁死亡
检测原理 |
WST-1可以和黄嘌呤氧化酶(Xanthine Oxidase,XO)催化产生的超氧化物阴离子(O2-)反应产生水溶性的甲臜染料(formazan dye),由于SOD能催化超氧化物阴离子发生歧化反应,所以该反应步骤可以被SOD所抑制,因此SOD的活性与甲臜染料的生成量呈负相关,通过对WST-1产物的比色分析即可计算SOD的活力。
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指标简称 | T-SOD |
灵敏度 | 0.2 U/mL |
检测范围 | 0.2-14.4 U/mL |
精密度 | 批间差:3.7% | 批内差:2.9% |
样本量 | 20 μL |
检测时间 | 30 min |
检测仪器及波长 | 酶标仪(440-460 nm) |
保存条件 | 该产品可在 2-8°C/-20°C避光条件下保存12个月,具体试剂组分的保存条件请参考说明书。 |
有效期 | 12个月 |
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Q1:每一次做实验都需要重新做对照孔和对照空白孔吗?
每次的实验环境、操作等影响因素都会有点差异,建议每次实验都重新做一下对照孔和空白孔,以便得到更准确的结果。
Q2:检测细胞样本SOD,用RIPA裂解液处理细胞行不行?
RIPA裂解液中SDS对酶活测定会有干扰,因此不建议使用RIPA裂解液进行组织或细胞的处理。SOD试剂盒要求样本中不能含有SDS、Tween20、NP-40、Triton X-100等去污剂,不能含有DTT、2-巯基乙醇等还原性试剂。
Q3:检测SOD,血液样本用EDTA抗凝行不行?
SOD的酶活中心为金属离子,用EDTA做抗凝剂会螯合金属离子,可能会造成SOD酶活降低,影响测值结果。
