Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

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中文名称：总超氧化物歧化酶（T-SOD）比色法测试盒（WST-1 法）

货号：E-BC-K020-M
文献（342）
说明书

说明书

Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

Price: ¥680.00 ¥380.00

Size:

96 T (92 samples) 48 T (44 samples)

样本类型： 血清（浆）;胸水;腹水;尿液;细胞;动植物组织
检测仪器及波长： 酶标仪（440-460 nm）
研究领域： 氧化应激;植物生理系列;铁死亡

为了简化计算过程，可下载该产品计算文件。如使用中有任何问题，请随时联系技术支持

该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M

说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证

根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮计算

检测原理	WST-1可以和黄嘌呤氧化酶（Xanthine Oxidase，XO）催化产生的超氧化物阴离子（O₂^-）反应产生水溶性的甲臜染料（formazan dye），由于SOD能催化超氧化物阴离子发生歧化反应，所以该反应步骤可以被SOD所抑制，因此SOD的活性与甲臜染料的生成量呈负相关，通过对WST-1产物的比色分析即可计算SOD的活力。
指标简称	T-SOD
灵敏度	0.2 U/mL
检测范围	0.2-14.4 U/mL
精密度	批间差：3.7% \| 批内差：2.9%
样本量	20 μL
检测时间	30 min
检测仪器及波长	酶标仪（440-460 nm）
保存条件	该产品可在 2-8°C/-20°C避光条件下保存12个月，具体试剂组分的保存条件请参考说明书。
有效期	12个月

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